Draft Genome Sequence of Bacillus thuringiensis Strain 907, an Isolate Toxic for Coleopteran / Projecto de Sequência Genómica de Bacillus thuringiensis Strain 907, um Tóxico Isolado para Coleopteran

Paulo Queiroz, Roberto Togawa, Priscila Grynberg, Érica Martins, Rose Monnerat


Bacillus thuringiensis is an important bactéria which shows insecticide action effective to control several Lepidoptera like Anticarsia gemmatalis, Helicoverpa armigera, Plutella xylostella and S. frugiperda, and Coleoptera like A. grandis. This work describes the draft genome sequence of B. thuringiensis S907, which contains the genes cry1Ba1, Vpb1Bc1, Vpa2Bb4 and Sip1Aa1. Genes coding for bacteriocin, plant growth promotion pathway and tyrosinase were identified.


cry toxin, boll weevil, ngs.


- Bravo A, Likitvivatanavong S, Gill SS, Soberón M. 2011. Bacillus thuringiensis: a story of a successful bioinsecticide. Insect Biochem Mol Biol. 41:423e431. doi: 10.1016/j.ibmb.2011.02.006.

- Portugal L, Munóz-Garay C, Martínez de Castro DL, Soberón M, Bravo A. 2017. Toxicity of Cry1A toxins from Bacillus thuringiensis to CF1 cells does not involve activation of adenylate cyclase/PKA signaling pathway. Insect Biochem Mol Biology. 80:21e31. doi: 10.1016/j.ibmb.2016.11.004.

- Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A. (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 28:1647-1649. doi: 10.1093/bioinformatics/bts199.

- Delcher AL, Kasif S, Fleischmann RD, Peterson J, White O, Salzberg SL. 1999. Alignment of whole genomes. Nucleic Acids Res. 27:2369–2376. doi.org/10.1093/nar/27.11.2369.

– Schattner P, Brooks AN, Lowe TM. 2005. The tRNAscan-SE, snoscan and snoGPS web servers for the detection of tRNAs and snoRNAs. Nucleic Acids Res. 33:W686-W689. doi: 10.1093/nar/gki366.

- Lagesen K, Hallin P, Rodland EA, Staerfeldt HH, Rognes T, Ussery DW. 2007. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res. 35:3100–3108. doi: 10.1093/nar/gkm160.

- Boeckmann B, Bairoch A, Apweiler R, Blatter MC, Estreicher A, Gasteiger E, Martin MJ, Michoud K, O’Donovan C, Phan I, Pilbout S, Schneider M. 2003. The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003. Nucleic Acids Res. 31:365–370. http:// dx.doi.org/10.1093/nar/gkg095. doi: 10.1093/nar/gkg095.

- Tatusov RL, Natale DA, Garkavtsev IV, Tatusova TA, Shankavaram UT, Rao BS, Kiryutin B, Galperin MY, Fedorova ND, Koonin EV. 2001. The COG database: new developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Res. 29:22–28. http:// dx.doi.org/10.1093/nar/29.4.e22. doi: 10.1093/nar/29.1.22.

- Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, Katayama T, Kawashima S, Okuda S, Tokimatsu T, Yamanishi Y. 2008. KEGG for linking genomes to life and the environment. Nucleic Acids Res. 36:D480–D484. http://dx.doi.org/10.1093/nar/gkm882.

- Quevillon E, Silventoinen V, Pillai S, Harte N, Mulder N, Apweiler R, Lopez R. 2005. InterProScan: protein domains identifier. Nucleic Acids Res. 33:W116 –W120. DOI: 10.1093/nar/gki442.

- Crickmore N, Berry C, Panneerselvam S, Mishra R, Connor TR, Bonning BC. A structure-based nomenclature for Bacillus thuringiensis and other bacteria- derived pesticidal proteins. Journal of Invertebrate Pathology. https://doi.org/10.1016/j.jip.2020.107438.

- Martins ES, Praça LB, Dumas VF, Silva-Werneck JO, Sone EH, Waga IC, Berry C, Monnerat RG. 2007. Characterization of Bacillus thuringiensis isolates toxic to cotton boll weevil (Anthonomus grandis). Biol Control. 40: 65–68. doi:10.1016/j.biocontrol.2006.09.009.

DOI: https://doi.org/10.34115/basrv6n3-008


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