Draft genome sequence of Bacillus thuringiensis strain S1287, an isolate showing insecticidal activity against coleoptera / Projeto de sequência do genoma de Bacillus thuringiensis strain S1287, um isolado mostrando actividade insecticida contra coleoptera

Paulo Queiroz, Roberto Togawa, Érica Martins, Priscila Grynberg, Rose Monnerat


Bacillus thuringiensis is an important bacterium showing insecticide proteins effective to control several important medical pests, including several Diptera species, like Diptera, Coleoptera and Lepidoptera. This work describes the draft genome sequence of B. thuringiensis S1287, which contains genes encoding the parasporal crystal cry, like Cry1Ab, Cry1Bb, Cry1C, Cry1D, Cry1Fb, Cry1Hb, Cry1Id, Cry1Ja, Cry1Ka and Cry1Nn. The genes belonging to the Vip Family were identified, Vpb1Aa, Vpa2Aa and Vip3Ag. Genes coding for plant growth promotion pathway were found.


insecticidal proteins, cry genes, boll weevil.


Ben-Dov E. 2014. Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins. Toxins. 6:1222–1243. doi: 10.3390/toxins6041222.

Martins ES, Praça LB, Dumas VF, Silva-Werneck JO, Sone EH, Waga IC, Berry C, Monnerat RG. 2007. Characterization of Bacillus thuringiensis isolates toxic to cotton boll weevil (Anthonomus grandis). Biol Control. 40:65–68. doi:10.1016/j.biocontrol.2006.09.009

- Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A. (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 28:1647-1649. doi: 10.1093/bioinformatics/bts199.

- Delcher AL, Kasif S, Fleischmann RD, Peterson J, White O, Salzberg SL. 1999. Alignment of whole genomes. Nucleic Acids Res. 27:2369–2376. doi: 10.1093/nar/27.11.2369.

– Schattner P, Brooks AN, Lowe TM. 2005. The tRNAscan-SE, snoscan and snoGPS web servers for the detection of tRNAs and snoRNAs. Nucleic Acids Res. 33:W686-W689. doi: 10.1093/nar/gki366.

- Lagesen K, Hallin P, Rodland EA, Staerfeldt HH, Rognes T, Ussery DW. 2007. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res. 35:3100–3108. doi: 10.1093/nar/gkm160.

- Boeckmann B, Bairoch A, Apweiler R, Blatter MC, Estreicher A, Gasteiger E, Martin MJ, Michoud K, O’Donovan C, Phan I, Pilbout S, Schneider M. 2003. The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003. Nucleic Acids Res. 31:365–370. http:// dx.doi.org/10.1093/nar/gkg095.

- Tatusov RL, Natale DA, Garkavtsev IV, Tatusova TA, Shankavaram UT, Rao BS, Kiryutin B, Galperin MY, Fedorova ND, Koonin EV. 2001. The COG database: new developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Res. 29:22–28. http:// dx.doi.org/10.1093/nar/29.4.e22.

- Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, Katayama T, Kawashima S, Okuda S, Tokimatsu T, Yamanishi Y. 2008. KEGG for linking genomes to life and the environment. Nucleic Acids Res. 36:D480–D484. http://dx.doi.org/10.1093/nar/gkm882.

- Quevillon E, Silventoinen V, Pillai S, Harte N, Mulder N, Apweiler R, Lopez R. 2005. InterProScan: protein domains identifier. Nucleic Acids Res. 33:W116 –W120. http://dx.doi.org/10.1093/nar/gni118.

– Crickmore N, Berry C, Panneerselvam S, Mishra R, Connor TR, Bonning BC. A structure-based nomenclature for Bacillus thuringiensis and other bacteria- derived pesticidal proteins. Journal of Invertebrate Pathology. https://doi.org/10.1016/j.jip.2020.107438

DOI: https://doi.org/10.34115/basrv6n3-009


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