Draft genome sequence of Bacillus thuringiensis strain S1905, an isolate toxic for lepidoptera / Projeto de sequência genómica de Bacillus thuringiensis strain S1905, um tóxico isolado para lepidoptera

Érica Martins, Priscila Grynberg, Roberto Togawa, Paulo Queiroz, Rose Monnerat


Bacillus thuringiensis Cry, Cyt, Vip and, Sip protein families show activity against insects of the orders Lepidoptera, Coleoptera and, Diptera. The Cry action toxins is to lyse midgut epithelial cells by forming pores in the target membrane. This work describes the draft genome sequence of B. thuringiensis S1905, which contains genes encoding Cry1A, Cry1I, Cry2A, Cry8K, and Vip3A. We found the spp1Aa gene and genes coding for plant growth promotion metabolic pathway and Thuricin 17. One gene encoding the toxin enhancin and a locus which encodes the CRISPR were identified.


plutella xylostella, ngs, shotgun.


Bravo A, Gill SS, Soberón M. 2007. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon. 49:423–435. doi: 10.1016/j.toxicon.2006.11.022.

– Sheppard AE, Poehlein A, Rosenstiel P, Liesegang H, Schulenburg H. 2013. Complete genome sequence of Bacillus thuringiensis strain 407 Cry-. Genome Announc. 1(1):e00158-12. doi:10.1128/genomeA.00158-12. doi:10.1128/genomeA.00158-12.

- Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A. (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics. 28:1647-1649. doi: 10.1093/bioinformatics/bts199.

- Delcher AL, Kasif S, Fleischmann RD, Peterson J, White O, Salzberg SL. 1999. Alignment of whole genomes. Nucleic Acids Res. 27:2369–2376. doi: 10.1093/nar/27.11.2369.

– Schattner P, Brooks AN, Lowe TM. 2005. The tRNAscan-SE, snoscan and snoGPS web servers for the detection of tRNAs and snoRNAs. Nucleic Acids Res. 33:W686-W689. doi: 10.1093/nar/gki366.

- Lagesen K, Hallin P, Rodland EA, Staerfeldt HH, Rognes T, Ussery DW. 2007. RNAmmer: consistent and rapid annotation of ribosomal RNA genes. Nucleic Acids Res. 35:3100–3108. doi: 10.1093/nar/gkm160.

- Boeckmann B, Bairoch A, Apweiler R, Blatter MC, Estreicher A, Gasteiger E, Martin MJ, Michoud K, O’Donovan C, Phan I, Pilbout S, Schneider M. 2003. The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003. Nucleic Acids Res. 31:365–370. http:// dx.doi.org/10.1093/nar/gkg095.

- Tatusov RL, Natale DA, Garkavtsev IV, Tatusova TA, Shankavaram UT, Rao BS, Kiryutin B, Galperin MY, Fedorova ND, Koonin EV. 2001. The COG database: new developments in phylogenetic classification of proteins from complete genomes. Nucleic Acids Res. 29:22–28. http:// dx.doi.org/10.1093/nar/29.4.e22.

- Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, Katayama T, Kawashima S, Okuda S, Tokimatsu T, Yamanishi Y. 2008. KEGG for linking genomes to life and the environment. Nucleic Acids Res. 36:D480–D484. http://dx.doi.org/10.1093/nar/gkm882.

- Quevillon E, Silventoinen V, Pillai S, Harte N, Mulder N, Apweiler R, Lopez R. 2005. InterProScan: protein domains identifier. Nucleic Acids Res. 33:W116 –W120. http://dx.doi.org/10.1093/nar/gni118.

– Crickmore N, Berry C, Panneerselvam S, Mishra R, Connor TR, Bonning BC. A structure-based nomenclature for Bacillus thuringiensis and other bacteria- derived pesticidal proteins. Journal of Invertebrate Pathology. https://doi.org/10.1016/j.jip.2020.107438

- Monnerat RG, Batista AC, Medeiros PT, Martins ES, Melatti VM, Praca LB, Dumas VF, Morinaga C, Demo C, Gomes ACM, Falcao R, Siqueira CB, Silva-Werneck JO, Berry C. 2007. Screening of Brazilian Bacillus thuringiensis isolates active against Spodoptera frugiperda, Plutella xylostella and Anticarsia gemmatalis. Biological Control 41:291–295. doi:10.1016/j.biocontrol.2006.11.008.

DOI: https://doi.org/10.34115/basrv6n3-012


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