A thermostable xylanase from a new strain of Aspergillus fumigatus presents high ability to hydrolyze hemicellulose from corn straw / Uma xilanase termoestável de uma nova estirpe de Aspergillus fumigatus apresenta elevada capacidade de hidrolisar hemicelulose a partir de palha de milho

Luciana Graciano, Kattlyn Laryssa Candido, Débora Jacomini, Bruna Simioni, Divair Christ, Marina Kimiko Kadowaki, José Luis da Conceição Silva, Caroline Henn, Alexandre Maller, Rita de Cássia Garcia Simão

Abstract


ABSTRACT

In order to optimize the production of xylanase from a new thermophilic strain of Aspergillus fumigatus (OI-1R-T), Plackett-Burman design (PBD) and central composite rotational design (CCRD) were performed. The response surface plots indicated a trend for increased xylanase biosynthesis with increasing concentrations of corn straw. The optimized xylanase activity was 530 U mL-1 in the presence of 6.5% (w/v) of the residual biomass, which was 11 times (1,157%) higher than that obtained with only the PBD (45.8 U mL-1). Interestingly, xylanase thermostability was maintained at 90% at 50 °C for 6 h. Enzymatic hydrolysis assays conducted for 96 h with 2 U mL-1 of xylanase and crude corn straw, pre-treated corn straw (hemicellulose) and xylan from beechwood, resulted in the net production of 3.89, 20.96 and 21.64 µmol mL-1of reducing sugars, respectively. Thus, A. fumigatus xylanase was equally able to hydrolyzes hemicellulose from corn straw and xylan from beechwood. The present data indicate that the xylanase activity of A. fumigatus could be applied to the production of low molecular weight sugars for use by pentose-fermenting yeast for the production of fuels and chemicals, among other products.

 

 


Keywords


Aspergillus fumigatus, corn straw, enzymatic hydrolysis, experimental design, xylanase, agro-industrial residues.

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DOI: https://doi.org/10.34117/bjdv6n9-377

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