Evaluation of the mutagenic potential in a mouse model of peripheral neuropathic pain / Avaliação do potencial mutagênico em um modelo de dor neuropática periférica em camundongos

Alexa Alves de Moraes, Walclécio Morais Lira, Renaly da Costa Rodrigues, Aline dos Santos DeMaman

Abstract


Objective: This study to evaluate the mutagenic potential of a neuropathic pain model induced by partial compressive ligation of the sciatic nerve after 30 hours, 7, 14, 42 and 70 days post-lesion, through micronucleus test. Methods: Eighteen male adult mice were equally divided into negative control group (NC); positive control group (PC), who received 50 mg/kg of cyclophosphamide; and partial sciatic nerve ligation group (SNL). In vivo micronucleus test in peripheral blood sample was performed by counting 2,000 polychromatic erythrocytes; It was carried out in NC at first; PC was tested after 30h of cyclophosphamide administration; SNL groups were tested 30 hours (SNL30h), 7 (SNL7d), 14 (SNL14d), 42 (SNL42d) and 70 (SNL70d) days post-lesion. Results:  Thirty hours after surgery, animals presented with an increased micronuclei frequency (5.89 ± 0.92), which slightly falls on the 7th post-lesion day. On the 14th day, however, the greatest mean of 8.11 ± 0.59 was attained, though another continuous decrease can be observed on both 42nd and 70th days post-injury. Discussion: Neuropathic pain induced by partial compressive ligation of the sciatic nerve is capable of triggering DNA damage, as suggested by the increase micronuclei incidence rates on the SNL groups.


Keywords


Neuropathy, Peripheral nerve injuries, Micronucleus test, Genomic instability, Pain, Sciatic nerve.

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References


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DOI: https://doi.org/10.34117/bjdv6n11-276

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