Orthodontic tooth movement in obese rats: preliminary histoenzymological results / Movimentação dentária ortodôntica em ratos obesos: um estudo preliminar

Authors

  • Ana Beatriz Campione Colombari Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Karla Carpio Horta
  • Erika Cavano Küchler
  • Fabio Lourenço Romano
  • Maria Bernadete Sasso Stuani
  • Caio Luiz Bitencourt Reis
  • Mirian Aiko Nakane Matsumoto

DOI:

https://doi.org/10.34117/bjdv7n4-232

Keywords:

Obesity, Resorption, Alveolar Bone Loss, Tooth Movement.

Abstract

The aim of this study is evaluated osteoclastogenesis on the periodontal ligament during induced tooth movement (ITM) in obese Wistar rats using the tartrate resistant acid phosphatase (TRAP) histoenzymatic analysis. Twelve rats weighing about 125 g were randomly divided into four groups of three animals each: Group I – healthy rats that received ITM in one side of the maxilla for seven days; Group II – obese rats that received ITM in one side of the maxilla for seven days; Group III – healthy rats that received ITM in one side of the maxilla for 14 days; Group IV – obese rats that received ITM in one side of the maxilla for 14 days. The other side of the maxilla was used as control side. Immediately after euthanasia, the samples were processed for histological examination using tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclasts in the periodontal ligament under conventional microscopy. Moderate to intense osteoclast activity was found in both sides of the maxilla that received ITM regardless of ITM duration or obesity of the Wistar rats. Our results suggest that obesity did not affect the formation of osteoclasts during orthodontic ITM.

References

WHO. Fact File: 10 Facts on Obesity. In: http://www.who.int/features/factfiles/obesity/facts/en/, editor. Geneva: World Health Organiz.

WHO. Fact sheet: Obesity and overweight. In: http://www.who.int/mediacentre/factsheets/fs311/en/, editor. Geneva: World Health Organiz; 2016.

WHO. UNICEF-WHO-The World Bank Group. Joint child malnutrition estimates – Levels and trends. In: http://www.who.int/nutgrowthdb/estimates2014/en, editor. Geneva: World Health Organiz; 2015.

Verna C, Zaffe D, Siciliani G. Histomorphometric study of bone reactions during orthodontic tooth movement in rats. Bone 1999;24:371-379.

Miyoshi K, Igarashi K, Saeki S, Shinoda H, Mitani H. Tooth movement and changes in periodontal tissue in response to orthodontic force in rats vary depending on the time of day the force is applied. Europ J Orthod 2001;23:329-338.

Hayashi H, Konoo T, Yamaguchi K. Intermittent 8-hour activation in orthodontic molar movement. Am J Orthod Dentofac Orthop 2004;125:302-309.

Dalla Vecchia CF, Susin C, R??sing CK, Oppermann RV, Albandar JM. Overweight and obesity as risk indicators for periodontitis in adults. J Period 2005;76:1721-1728.

Pischon N, Heng N, Bernimoulin J-P, Kleber B-M, Willich S, Pischon T. Obesity, inflammation, and periodontal disease. J Dent Res 2007;86:400-409.

Priesnitz Simch R, Jose Gaio E, Kuchenbecker Rösing C. Effect of body weight in the pathogenesis of ligature-induced periodontal disease in Wistar rats. Acta Odont Scand 2008;66:130-134.

Verzeletti GN, Gaio EJ, Linhares DS, Rösing CK. Effect of obesity on alveolar bone loss in experimental periodontitis in Wistar rats. J App Oral Sci2012;20:218-221.

Jordani P, Campi L, Circeli G, Visscher C, Bigal M, Gon?ßalves D. Obesity as a risk factor for temporomandibular disorders. J Oral Rehab 2017;44:1-8.

Dündar S, Yaman F, Ozupek MF, Saybak A, Gul M, Asutay F et al. The effects of high-fat diet on implant osseointegration: an experimental study. J Korean Assoc Oral Maxillofac Surg 2016;42:187-192.

Alswat K, Mohamed WS, Wahab MA, Aboelil AA. The association between body mass index and dental caries: Cross-sectional study. J Clinical Med Res 2016;8:147.

Costacurta M, Sicuro L, Di Renzo L, Condò R, De Lorenzo A, Docimo R. Childhood obesity and skeletal-dental maturity. Eur J Paediatr Dent 2012;13:128-132.

Akridge M, Hilgers KK, Silveira AM, Scarfe W, Scheetz JP, Kinane DF. Childhood obesity and skeletal maturation assessed with Fishman's hand-wrist analysis. Am J Orthod Dentofac Orthop 2007;1 32:185-190.

Mack KB, Phillips C, Jain N, Koroluk LD. Relationship between body mass index percentile and skeletal maturation and dental development in orthodontic patients. Am J Orthod Dentofac Orthop 2013; 143:228-234.

Hedayati Z, Khalafinejad F. Relationship between Body Mass Index, Skeletal Maturation and Dental Development in 6-to 15-Year d Orthodontic Patients in a Sample of Iranian Population. J Dent 2014;15:180.

Must A, Phillips SM, Tybor DJ, Lividini K, Hayes C. The association between childhood obesity and tooth eruption. Obesity 2012;20:2070-2074.

Santamaria M, Milagres Db, Stuani AS, Stuani MBS, de Oliveira RuellasAnC. Initial changes in pulpal microvasculature during orthodontic tooth movement: a stereological study. Europ J Orthod 2006;28:217-220.

Cavagni J, de Macedo IC, Gaio EJ, Souza A, de Molon RS, Cirelli JA et al. Obesity and hyperlipidemia modulate alveolar bone loss in Wistar rats. J Periodont 2016;87:e9-e17.

Estadella D, Oyama LM, Dâmaso AR, Ribeiro EB, Do Nascimento CMO. Effect of palatable hyperlipidic diet on lipid metabolism of sedentary and exercised rats. Nutrition 2004; 20:218-224.

Heller IJ, Nanda R. Effect of metabolic alteration of periodontal fibers on orthodontic tooth movement: an experimental study. Am J Orthod 1979;75:239-258.

Martins-Ortiz MF. Influência dos bisfosfonatos na movimentação dentária induzida, na frequência e nas dimensões das reabsorções radiculares associadas: Universidade de São Paulo; 2004.

Miyoshi K, Igarashi K, Saeki S, Shinoda H, Mitani H. Tooth movement and changes in periodontal tissue in response to orthodontic force in rats vary depending on the time of day the force is applied. Europ J Orthod 2001;23:329-338.

Consolaro A. Induced tooth movement: applied biology to the clinical practice. Maringá: Dent Press J Orthod; 2002.

M. Nishimura, M. Chiba, T. Ohashi, M. Sato, Y. Shi- mizu, K. Igarashi, and H. Mitani, Periodontal tissue activation by vibration: Intermittent stimulation by re- sonance vibration accelerates experimental tooth movement in rats. Am J Orthod Dentofac Orthop 133, 572–583 (2008).

T. Kim, A. Handa, J. Iida, and S. Yoshida, RANKL expression in rat periodontal ligament subjected to a continuous orthodontic force. Arch Oral Biol.52, 244–250 (2007).

Garcia VG, Longo M, Gualberto Júnior EC, Bosco AF, Nagata MJ, Ervolino E, Theodoro LH (2014) Effect of the concentration of phenothiazine photosensitizers in antimicrobial photodynamic therapy on bone loss and the immune inflammatory response of induced periodontitis in rats. J Period Res 49:584–594

Kim YD, Kim SS, Hwang DS et al (2007) Effect of low-level laser treatment after installation of dental titanium implant- immunohistochemical study of RANKL, RANK, OPG: an experi- mental study in rats. Lasers Surg Med 39:441–450

Michelogiannakis D, Rossouw PE, Khan J, Akram Z, Menenakos E, Javed F. "Influence of increased body mass index on orthodontic tooth movement and related parameters in children and adolescents: A systematic review of longitudinal controlled clinical studies." . 2019 Sep 14; :1465312519873669. Epub 2019 Sep 14.

Chaves VE, Frasson D, Martins-Santos ME, Boschini RP, Garófalo MA, Festuccia WT et al. Glyceroneogenesis Is Reduced and Glucose Uptake Is Increased in Adipose Tissue from Cafeteria Diet-Fed Rats Independently of Tissue Sympathetic Innervation. J Nutrit 2006;136:2475-2480.

Andreollo NA, Santos EFd, Araújo MR, Lopes LR. Rat's age versus human's age: what is the relationship? ABCD. Arq Bras Cirurg Digest (São Paulo) 2012;25:49-51.

Dietrich MO, Muller A, Bolos M, Carro E, Perry ML, Portela LV et al. Western style diet impairs entrance of blood-borne insulin-like growth factor-1 into the brain. Neuromolecular medicine 2007;9:324-330.

Bartolomucci A, Cabassi A, Govoni P, Ceresini G, Cero C, Berra D et al. Metabolic consequences and vulnerability to diet-induced obesity in male mice under chronic social stress. PLoS One 2009;4:e4331.

Knight SF, Quigley JE, Yuan J, Roy SS, Elmarakby A, Imig JD. Endothelial dysfunction and the development of renal injury in spontaneously hypertensive rats fed a high-fat diet. Hypertension 2008;51:352-359.

Damanaki A, Nokhbehsaim M, Hiththetiya K, Memmert S, Gao J, Nguyen KA, Götz W, Jäger A, Wahl G, Deschner J. Characterization of a diet-induced obesity rat model for periodontal research. Clin Oral Investig. 2018. https://doi.org/10.1007/s00784-018-2514-y.

Zhao L, Matsumoto Y, Ono T, Iseki S.. Effects of mechanical force application on the developing root apex in rat maxillary molars. Arch Oral Biol. 2019.05; 101 64-76.

Chisari JR, McGorray SP, Nair M and Wheeler TT (2014) Variables affecting orthodontic tooth movement with clear aligners. Am J Orthod Dentofac Orthop 145(4 Suppl): S82–91.

Published

2021-04-09

How to Cite

Colombari, A. B. C., Horta, K. C., Küchler, E. C., Romano, F. L., Stuani, M. B. S., Reis, C. L. B., & Matsumoto, M. A. N. (2021). Orthodontic tooth movement in obese rats: preliminary histoenzymological results / Movimentação dentária ortodôntica em ratos obesos: um estudo preliminar. Brazilian Journal of Development, 7(4), 36685–36698. https://doi.org/10.34117/bjdv7n4-232

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Section

Original Papers