Effect of nutritional factors on the Aspergillus nidulans germination program / Efeito de fatores nutricionais sobre o programa de germinação de conídios de Aspergillus nidulans

Authors

  • Gisele Arruda Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Izabel Aparecida Soares
  • Carmem Lúcia de Mello Sartori Cardoso da Rocha

DOI:

https://doi.org/10.34117/bjdv7n4-322

Keywords:

Nutritional constituents. Filamentous fungus. Culture media. Germination of conidia.

Abstract

The development of conidia germination of the filamentous fungus Aspergillus nidulans can be used to evaluate the effects of different agents. The objective of the present work was to test eight compositions of the culture medium for the germination of conidia of the bimeth strain. Dormant conidia were inoculated in minimal medium with the following compositions: TN1 (MM + glyc 1% + VIT), TN2 (MM + glyc 0.1% + VIT), TN3 (MM + glyc 1% + VIT + casein), TN4 (MM + 0.1% gli + VIT + casein), TN5 (KH2PO4 + 1% gli + VIT), TN6 (KH2PO4 + 1% gli + casein), TN7 (NaNO3 + KCl, 1% gli + VIT) and TN8 (MC). Five hundred conidia were analyzed in each observation, determining the percentage of conidia in each germination phase, for a period of up to 24 hours. After that time, the percentages of death and malformation of the conidia were determined. The results showed changes in germinative development, in the generation of dead and malformed in all TNs that lacked nutrients, when compared to MM and MC. When testing two glucose concentrations, it can be seen that glucose is not consumed during germination, although it is necessary for breaking dormancy. The absence of casein showed that the bimeth strain, which has a requirement for methionine, germinated in the absence of methionine, therefore, it was able to synthesize at least a small amount of this amino acid, although in casein media the germination was better. The absence of phosphate totally inhibited the germination of the conidia, due to the lack of raw material for DNA duplication. Some media that are poorer in nutrients than MM have shown to allow germination. The analysis of dead and malformed showed that TNs 3, 4 and 6 had a disrupting effect on polarized growth and TNs 1, 2 and 5 had a more drastic inhibitory effect, increasing cell death. The results of this work show that the conidia had changes in the development of germination in response to nutritional conditions, showing that the choice of constituents for the preparation of the medium to be used influences the responses of germination, death and malformations in conidia of A. nidulans.

References

Abdel-Rahim AM and Arbab HA (1985) Nutrient requirements in germination of conidiospores of Aspergillus niger V. Tieghen. Mycopathologia 92: 111-113. https://pubmed.ncbi.nlm.nih.gov/4079968/

Alves GF Solubilização do fosfato de rocha por Aspergillus niger. 146f. Dissertação Mestrado em Engenharia Química. Universidade Federal de Uberlândia. Faculdade de Engenharia Química. Uberlândia, 2012.

Amable P and Butler M (2008) Cell metabolism and its control in culture. In: Castilho L, Moraes A, Augusto E and Butler M (edt). Animal Cell Technology: From Biopharmaceuticals to Gene Therapy, Taylor & Francis, pp. 75-110.

D´Enfert C (1997) Fungal spore germination: insights from the molecular genetics of Aspergillus nidulans and Neurospora crassa. Fungal Genetics and Biology 21:163-172. https://www.sciencedirect.com/science/article/abs/pii/S1087184597909750

Elbein AD (1974) The metabolism of ?, ?-trehalose. Adv. Carbohydr. Chem. Biochem 30:256-277. https://pubmed.ncbi.nlm.nih.gov/4377836/

Gottlieb D (1950) The Physiology of Spore Germination in Fungi Botanical Review 16: 229-257. https://link.springer.com/article/10.1007/BF02873609

Griffin DH (1994) Fungal physiology. 2 nd ed. New York: Willey-Liss, pp. 134-136.

Hassouni H, Ismaili-Alaoui1 M, Lamrani K., Gaime-Perraud I, Augur C and Roussos S (2007) Comparative spore germination of filamentous fungi on solid state fermentation under different culture conditions. Micologia Aplicada International 19: 7-15. https://webcache.googleusercontent.com/search?q=cache:yVlIBHdKJ4oJ:https://www.imbe.fr/docrestreint.api/1091/068989f49904a3ad53f5345f286b4401f9135a05/pdf/pdf_32_hassouni_et_al_2007.pdf+&cd=3&hl=pt-BR&ct=clnk&gl=br&client=safari

Hayer K, Stratford M and Archer DB (2014) Germination of Aspergillus niger conidia is triggered by nitrogen compounds related to L-Amino acids. Applied and Environmental Microbiology 80: 6046-6053. https://www.semanticscholar.org/paper/Germination-of-Aspergillus-niger-Conidia-Is-by-to-Hayer-Stratford/bf914ef94eab22f46655f7fa8c4c2ced4a2a34bc

Hottiger T, De Virgilio C, Hall MN, Boller T and Wiemken A (1994) The role of trehalose synthesis for the acquisition of thermotolerance in yeast. II. Physiological concentrations of trehalose increase the thermal stability of proteins in vitro. Eur. J. Biochem 219:187-193. https://pubmed.ncbi.nlm.nih.gov/8306985/

Kap-Hoon H, Dong-Beom L, Jong-Hak K, Min-Su K, Kyu-Yong H, Won-Shin K, Young-Soon P, Heui-Baik K and Dong-Min H (2003) Environmental factors affecting development of Aspergillus nidulans. The Journal of Microbiologyarch 41: 34-40.

Lin CK (1940) Germination of conidia of Sclerotinia fructicola with special reference to the toxicity of copper. Cornell Univ. Agr. Exp. Sta 233:1-30.

Lin CK (1945) Nutrient requirements in the germination of the conidia of Glomerella cingtulata. Am. Jour. Bot 6:296-298.

Mcdowell LR Minerals in Animal and Human Nutrition. Ed. L. R. McDowell. Academic Press. pg 27-77. New York, 1992.

Nelson EB (1991) Exudate molecules initiating fungal responses to seeds and roots. In: Keister L and Cregan PB (eds) The Rhizoshere and Plant Growth. Amsterdam, pp.197-209. https://link.springer.com/article/10.1007/BF00011692

Nelson EB and Hsu JS (1994) Nutritional factors affecting responses of sporangia of Pythium ultimum to germination stimu lants. Phytopathology 84:677-683. https://europepmc.org/article/agr/ind20443166

Osherov N and May GS (2000) Conidial germination in Aspergillus nidulans requires RAS signaling and protein synthesis. Genet 155: 647-656. https://pubmed.ncbi.nlm.nih.gov/10835388/

Pass T and Griffin GJ (1972) Exogenous carbon and nitrogen requirements for conidial germination by Aspergillus flavus. Canadian Journal of Microbiology 18: 1453-1461. https://pubmed.ncbi.nlm.nih.gov/4627197/

Pontecorvo G, Roper JA, Hemmons LM and Bufton AWJ (1953) The Genetics Of Aspergillus Nidulans. Advances in Genetics 5:141-238. https://www.sciencedirect.com/science/article/abs/pii/S0065266008604083

Pulz P and Massola Junior NS (2009) Efeito de meios de cultura e fatores físicos no crescimento e esporulação de Alternaria dauci e A. solani. Summa phytopathol 35:121-126. https://www.scielo.br/scielo.php?pid=S0100-54052009000200007&script=sci_abstract&tlng=pt

Ryan FJ (1948) The germination of conidia from biochemical mutants of Neurospora. Am. Jour. Bot 35: 497-503. https://bsapubs.onlinelibrary.wiley.com/doi/abs/10.1002/j.1537-2197.1948.tb08112.x

Schmit JC and Brody S (1976) Biochemical genetics of Neurospora crassa conidial germination. Bacteriol. Rev 40:1-41. https://pubmed.ncbi.nlm.nih.gov/5072/

Shimoda C (1980) Differential effect of glucose and fructose on spore germination in the fission yeast, Schizosaccharomyces pombe. Can. J. Microbiol 26:741-745. https://cdnsciencepub.com/doi/10.1139/m80-129

Sudbery PE (2008) Regulation of polarised growth in fungi. Fungal biology reviews 22: 44-55. https://www.sciencedirect.com/science/article/abs/pii/S1749461308000213

Szyf M (2011) The implications of DNA methylation for toxicology: toward toxicomethylomics, the toxicology of DNA methylation. Toxicol. Sci. Orlando 120:235-155. https://pubmed.ncbi.nlm.nih.gov/21297083/

Taheri-Talesh N, Horio T, Araujo-Bazán L, Dou X, Espeso EA, Peñalva MA, Osamani AS and Oaklet BR (2008) The tip growth apparatus of Aspergillus nidulans. Molecular Biology of the Cell 19: 1439-1449. https://pubmed.ncbi.nlm.nih.gov/18216285/

Thevelein JM (1996) Regulation of trehalose metabolism and its relevance to cell growth and function. In: Brambland R and Marzluf GA (eds.) The Mycota. III. Biochemistry and Molecular Biology. Springer-Verlag, Berlin, pp. 395-420. https://link.springer.com/chapter/10.1007/978-3-662-10367-8_19

Timberlake WE and Clutterbuck AJ (1994) Genetic regulation of conidiation. In: Martinelli SD and Kinghorn JR (eds.) Aspergillus: 50 years on. Elsevier, New York, pp. 383-407. https://pubmed.ncbi.nlm.nih.gov/7765135/

Timberlake WE and Marshall MA (1988) Genetic regulation of development in Aspergillus nidulans. Trends in Genetics 4: 162-169. https://pubmed.ncbi.nlm.nih.gov/3076298/

Uppal BN (1924) Spore germination of Phytophthora infestans. Phyto- path 14:32-33.

Wenzel IM, Monteiro AC and Pereira GT (2007) Desempenho de Lecanicillium lecanii em meios de cultura contendo vitaminas e concentrações de extrato de levedura. Bragantia 66: 413-421. https://www.scielo.br/scielo.php?pid=S0006-87052007000300007&script=sci_abstract&tlng=pt

Yamada T and Abdalla SRR Fósforo na agricultura brasileira. Associação Brasileira para Pesquisa da Potassa e do Fosfato. Piracicaba, 2004. https://www.npct.com.br/npctweb/npct.nsf/e0f085ed5f091b1b852579000057902e/eacab2541ec728830325844f0074968f/$FILE/Fósforo%20na%20Agricultura%20Brasileira%20-%20Sumário.pdf

Published

2021-04-13

How to Cite

Arruda, G., Soares, I. A., & da Rocha, C. L. de M. S. C. (2021). Effect of nutritional factors on the Aspergillus nidulans germination program / Efeito de fatores nutricionais sobre o programa de germinação de conídios de Aspergillus nidulans. Brazilian Journal of Development, 7(4), 38071–38089. https://doi.org/10.34117/bjdv7n4-322

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Section

Original Papers