Morphological and physiological characterization and biomass production of Burkholderia pyrrocinia (BRM 32113) / Caracterização morfológica e fisiológica e produção de biomassa de Burkholderia pyrrocinia (BRM 32113)

Marina Teixeira Arriel Elias, Maythsulene Inácio de Sousa Oliveira, Gustavo Andrade Bezerra, Marcio Vinicius de Carvalho Barros Côrtes, Marta Cristina Corsi de Filippi

Abstract


We aimed to evaluate the viability of agroindustry residues for Burkholderia pyrrocinia (BRM32113) biomass production. BRM32113 was morphological and physiologically characterized; carbon: nitrogen ratio, optimal pH and osmotic conditions were determined and carbon sources profile analyzed. Biomass production was tested with glycerol, molasses, and rice brain, and quantification was obtained by counting colony-forming unity (CFU). BRM32113 cells are bacilli shaped, and the colony is dark pink gram-negative with high capacity to assimilate carbon sources, producing 8.9E+10 CFU.mL-1 in glycerol-containing medium. Optimal C:N was from 2.5 to 3.5, pH ranged from 8.0 to 9.5, and the maximum growth was obtained in period from 6 to 8 hours. The growth rate was 0.332 h-1, and the optimal NaCl concentration was 1 and 2%. Crude glycerol as carbon source was superior. We proved that it is viable to produce biomass from agroindustry waste.


Keywords


agribusiness, bacteria, bioproduct, prospecting, waste.

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DOI: https://doi.org/10.34117/bjdv7n4-529

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