Evaluation of the mineralizing capacity of varnishes by electrical impedance spectroscopy: an in vitro study / Avaliação da capacidade mineralizante de vernizes por espectroscopia de impedância elétrica: um estudo in vitro

Authors

  • Maurício Vaz Munhão
  • João Victor Frazão Câmara
  • Ana Clara Correa Duarte Simões
  • Marijoe Braga Alves Simões
  • Renato Feres de Carvalho Vianna
  • Denise Fernandes Lopez Nascimento
  • Maria do Céu Pinto do Amaral
  • Angela Alexandre Meira Dias
  • Sonia Groisman

DOI:

https://doi.org/10.34117/bjdv.v7i5.29365

Keywords:

Dental Caries, Chlorhexidine, Demineralization, Fluorides, Topical, Electric Impedance.

Abstract

Background: To evaluate in vitro and compare a remineralizing capacity of three different types of varnishes. Material and Methods: The sample consisted of 24 third molars found in cycles of demineralization and remineralization (DES X RE) and distributed in three groups: Group 1 - fluoride varnish (NaF 5%; Duraphat, Colgate, São Paulo, Brazil), Group 2 - casein phosphopeptide varnish - amorphous calcium phosphate (CPP-ACP 10%) (Recaldent GC, Tokyo, Japan), Group 3 - 1% chlorhexidine varnish (Cervitec® Plus - Ivoclar Vivadent), applied to the mesial and distal as control. The mineral content of the enamel was evaluated using the CarieScan ProTM, at the central point of the sites at three times: before the DES X RE cycle, after it and after using the varnishes. Results: The results were obtained from the descriptive analysis of data using the Excel tool. In G1, the sodium fluoride varnish, Duraphat, showed the best effectiveness in the remineralization process, which occurred in 75% of the sites, followed by G2, which remineralized 50% of the sites and G3, which was less efficient, remineralizing only 31.25% of the sites. Conclusion: There is variation in the remineralizing capacity among the varnishes studied. The varnish based on 5% sodium fluoride, Duraphat, showed the best effectiveness in the remineralization process, also acting at a distance.

References

Silva TFA, Feitosa JL, Dantas RMM, Medeiros FCD, Lima IPC, Seabra EJG. Modelo experimental de cárie dentária como ferramenta educativa em escolares. Rev. salud pública. 2016;(2):290-9.

Chau NPT, Pandit S, Jung J, Jeon J. Evaluation of Streptococcus mutans adhesion to fluoride varnishes and subsequent change in biofilm accumulation and acidogenicity. J Dent. 2014;42(3):726–34.

Aziznezhad M, Alaghemand H, Shahande Z, Pasdar N, Bijani A, Eslami A, Dastan Z. Comparison of the effect of resin infiltrant, fluoride varnish, and nano-hydroxy apatite paste on surface hardness and streptococcus mutans adhesion to artificial enamel lesions. Electronic Physician. 2017;9(3):3934-942.

Alkilzy M, Santamaria RM, Schmoeckel J, Splieth CH. Treatment of Carious Lesions Using Self-Assembling Peptides. Adv Dent Res. 2018;29(1):42–7.

Alexandria AK, Nassur C, Nóbrega CBC, Valença AMG, Rosalen PL, Maia LC. In situ effect of titanium tetrafluoride varnish on enamel demineralization. Braz Oral Res. 2017;31:e86.

Sanabe ME, Motisuki C, Negrini TC, Spolidorio DMP, Hebling J. Efeito da Aplicação da Clorexidina no Controle de Estreptococos Mutans do Biofilme Oclusal. 24 Braz J Health. 2010 ;1(1):37-46.

Mathur VP, Dhillon JK. Dental Caries: A Disease Which Needs Attention. Indian J Pediatr. 2018;85(3):202-206.

Kutsch VK. Dental caries: an updated medical model of risk assessment. J Prosthet Dent. 2014;111(4):280-5.

Oliveira PRA, Barboza CM, Barreto LSDC, Tostes MA. Effect of CPP-ACP on remineralization of artificial caries-like lesion: an in situ study. Braz Oral Res. 2020;34:e061.

Varma V, Hegde KS, Bhat SS, Sargod SS, Rao HA. Comparative Evaluation of Remineralization Potential of Two Varnishes Containing CPP-ACP and Tricalcium Phosphate: An In Vitro Study. Int J Clin Pediatr Dent. 2019;12(3):233-236.

Melo M, Pascual A, Camps I, Del Campo A. In vivo study of different methods for diagnosing pit and fissure caries. J Clin Exp Dent. 2015;7(3):387-91.

Queiroz CS. Modelos de estudos in vitro para avaliar o efeito do fluoreto na desmineralização e remineralização do esmalte e dentina. Tese de doutorado Piracicaba, Universidade Estadual de Campinas. 2004. 79p.

Langhorst SE, O'Donnell JNR, Skrtic D. In vitro remineralization of enamel by polymeric amorphous calcium phosphate composite: Quantitative micro-radiographic study. Dent Mater. 2009;25(7):884–91.

Yu OY, Zhao IS, Mei ML, Lo EC, Chu CH. A Review of the Common Models Used in Mechanistic Studies on Demineralization-Remineralization for Cariology Research. Dent J (Basel). 2017;5(2):20.

Neuhaus KW, Schlafer S, Lussi A, Nyvad B. Infiltration of Natural Caries Lesions in Relation to Their Activity Status and Acid Pretreatment in vitro. Caries Res. 2012;47(12):203–10.

Mohd Said SN, Ekambaram M, Yiu CK. Effect of different fluoride varnishes on remineralization of artificial enamel carious lesions. Int J Paediatr Dent. 2017;27(3):163-173.

Agrawal N, Shashikiran ND, Singla S, Ravi KS, Kulkarn VK. Atomic force microscopic comparison of remineralization with caseinphosphopeptide amorphous calcium phosphate paste, acidulated phosphate fluoride gel and iron supplement in primary and permanent teeth: An in vitro study. Contemp Clin Dent. 2014?5(1):75–80.

Thakkar PJ, Badakar CM, Hugar SM, Hallikerimath S, Patel PM, Shah P. An in vitro comparison of casein phosphopeptide-amorphous calcium phosphate paste, casein phosphopeptide-amorphous calcium phosphate paste with fluoride and casein phosphopeptide-amorphous calcium phosphate varnish on the inhibition of demineralization and promotion of remineralization of enamel. J Indian Soc Pedod Prev Dent. 2017;35(4):312-318.

Kühnisch J, Heinrich-Weltzien R, Tabatabaie M, Stösser L, Huysmans MC. An in vitro comparison between two methods of electrical resistance measurement for occlusal caries detection. Caries Res. 2006;40(2):104-11.

Horst JA, Tanzer JM, Milgrom PM. Fluorides and Other Preventive Strategies for Tooth Decay. Dent Clin North Am. 2018;62(2):207-234.

Carvalho DM, Salazar M, Oliveira BH, Coutinho ESF. O uso de vernizes fluoretados e a redução da incidência de cárie dentária em pré-escolares: uma revisão sistemática Fluoride varnishes and decrease in caries incidence in preschool children: a systematic review. Rev Bras Epidemiol. 2010;13(1):139–49.

Thierens LAM, Moerman S, Elst CV, et al. The in vitro remineralizing effect of CPP-ACP and CPP-ACPF after 6 and 12 weeks on initial caries lesion. J Appl Oral Sci. 2019;27:e20180589.

Memarpour M, Fakhraei E, Dadaein S, Vossoughi M. Efficacy of fluoride varnish and casein phosphopeptide-amorphous calcium phosphate for remineralization of primary teeth: a randomized clinical trial. Med Princ Pract. 2015;24(3):231-237.

Wang Y, Li J, Sun W, Li H, Cannon RD, Mei L. Effect of non-fluoride agents on the prevention of dental caries in primary dentition: A systematic review. Plos One. 2017 Aug;12(8) e0182221.

Cochrane NJ, Shen P, Yuan Y, Reynolds EC. Ion release from calcium and fluoride containing dental varnishes. Australian Dental Journal. 201459(1):100–5.

Published

2021-06-07

How to Cite

Munhão, M. V., Câmara, J. V. F., Simões, A. C. C. D., Simões, M. B. A., Vianna, R. F. de C., Nascimento, D. F. L., Amaral, M. do C. P. do, Dias, A. A. M., & Groisman, S. (2021). Evaluation of the mineralizing capacity of varnishes by electrical impedance spectroscopy: an in vitro study / Avaliação da capacidade mineralizante de vernizes por espectroscopia de impedância elétrica: um estudo in vitro. Brazilian Journal of Development, 7(5), 45110–45123. https://doi.org/10.34117/bjdv.v7i5.29365

Issue

Section

Original Papers