Multiplex PCR for molecular characterization of isolated strains of granuloma in sheep from state of Sergipe and Antimicrobial Susceptibility Test (AST) / PCR Multiplex para caracterizaçao molecular de linhagem isolada de granuloma em ovelha no estado de Sergipe e Teste de suceptibilidade antimicrobiana

Authors

  • Lumar Lucena Alves Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Daniela Droppa Almeida
  • Caroline de Santana Ferreira
  • Ioná Brito
  • Glenda Amaral da Silva
  • Lívia Maria do Amorim Costa Gaspar
  • Afonso Celso Dórea
  • Francine Ferreira Padilha

DOI:

https://doi.org/10.34117/bjdv.v7i5.29869

Keywords:

Corynebacterium pseudotuberculosis, Caseous Lymphadenitis, Granuloma

Abstract

Corynebacterium pseudotuberculosis is the etiologic agent responsible for Caseous Lymphadenitis (CLA), an infectious disease that affects cattle and horses, but mostly small ruminants such as sheep and goats. This pathology, also known as the "bad seed," causes abscesses formation in the contaminated animal tissue, which can be superficial or visceral. As it is present in several Brazilian states, it is necessary to find out effective ways of diagnostic, prophylactic and effective treatment for CLA in attempt to eliminate this pathogen in rural areas. In Sergipe, the goat farming has progressively gained recognition in recent years and has surpassed over 50% of sheep flocks in the last decade. Therefore, Sergipe has presented a considerable development in sheep farming as well as it has expanded its potential commercialization regarding these animals and their derivatives. However, with the lack of epidemiological analysis, it becomes difficult to know both the presence and prevalence of the disease in the state. Thus, this study aimed to identify the microorganism isolated from a sheep granuloma in Sergipe not only by biochemical tests, considered the gold standard for C. pseudotuberculosis but also by molecular analysis through multiplex PCR, as well as to evaluate the antimicrobial susceptibility test.

References

ABREU SRO, MOTA RA, JÚNIOR JWP, ROSINHA GMS, CASTRO RS (2008). Perfil de sensibilidade antimicrobiana in vitro de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos com linfadenite caseosa no sertão de Pernambuco, v. 15(3), 502- 509.

AL-HARBI, Khaled Bani (2001). Prevalence and etiology abscesso disease of sheep and goats at Qassim regioin, Saudi Arabia. Veterinary world; v.4(11), 495-499.

ALVES FSF, LAUANA BS, RAYMUNDO RP (2007). Linfadenite caseosa: o estado da arte. Documentos, Embrapa Caprinos; Sobral, 1-60.

ASCCO, Associação Sergipana de Criadores e Ovinos. Exposição Nacional da Raça Santa Inês. Available at: www.ascco.com.br. Acessed 23 May 2016.

BAUER AW, KIRBY WMM, SHERRIS JC, TURCK M (1966). Antibiotic susceptibility testing by standardized single disk method. American Journal of Clinical Pathology; v. 45, 493-496.

BELCHIOR SE, GALLARDO A, ABALOS A, JODOR N, JENSEN O (2006). Actualizacion sobre linfoadenitis caseosa: el agente etiológico y la enfermedad. Revista Veterinaria Argentina; v. 23 (224), 258-78.

CONNOR KM, QUIRIE MM, BAIRD G, DONACHIE W (2000). Characterization ?Kingdom isolates of Corynebacterium pseudotuberculosis using pulsed-field gel electrophoresis, Journal of Clinic Microbiology; v. 38, 2633– 2637.

DORELLA F. PACHECO LGC, OLIVEIRA S, MIYOSHI A, AZEVEDO V (2006). Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence. Veterinary Research; v. 37(2), 201-18.

DORELLA FA, GALA-GARCIA A, PINTO AC, SARROUH B, ANTUNES CA, RIBEIRO D, ABURJAILE FF, FIAUX KK, GUIMARÃES LC, SEYFFERT N, EL-AOUAR RA, SILVA R, HASSAN SS, CASTRO TLP, MARQUES WS, RAMOS R, CARNEIRO A, DE SÁ P, MYIOSHI A, AZEVEDO V, SILVA A (2013). Progression of 'OMICS' methodologies for understanding the pathogenicity of Corynebacterium pseudotuberculosis: the Brazilian experience. Computational and Structural Biotechnology Journal; v. 6, 1 – 7.

DROPPA-ALMEIA D, VIVAS WL, SILVA KK, SIMIONATTO S, MEYER R, LIMA-VERDE IB, DELAGOSTIN O, BORSUK S, PADILHA FF (2016). Recombinant CP40 from Corynebacterium pseudotuberculosis confers protection in mice after challenge with a virulent strain. Vaccine; v.34, 1091–1096.

GUIMARÃES AS, CARMO FB, PAULETTI RB, SEYFFERT N, RIBEIRO D, LAGE AP, HEINEMANN MB, MIYOSHI A, AZEVEDO V, GOUVEIA AMG (2011). Caseous Lymphadenitis: epidemiology, diagnosis and control. The IIOAB jornal; v.2 (2), 33-43.

GUEDES MT, SOUZA BC, SOUSA TJ, LOUREIRO D, MOURA-COSTA LF, AZEVEDO V, MEYER R, PORTELA RW (2015). Infecção por Corynebacterium pseudotuberculosis em equinos: aspectos microbiológicos, clínicos e preventivos. Pesquisa Veterinária Brasileira; v.35 (8), 701-708.

HASSAN SS, GUIMARÃES LC, PEREIRA UP, ISLAM A, ALI A, BAKHTIAR SM, RIBEIRO D, SANTOS AR, SOARES SC, DORELLA F, PINTO AC, SCHNEIDER MPC, BARBOSA MS, ALMEIDA S, ABREU V, ABURJAILE F, CARNEIRO AR, CERDEIRA LT, FIAUX K, BARBOSA E, DINIZ C, ROCHA FS, RAMOS RTJ, JAIN N, TIWARI S, BARH D, MIYOSHI A, MULLER B, SILVA A, AZEVEDO V (2012). Complete genome sequence of Corynebacterium pseudotuberculosis biovar ovis strain P54B96 isolated from antelope in South Africa obtained by rapid next generation sequencing technology. Standards in Genomic Science; v.7, 189-199.

IBGE. Instituto Brasileiro de Geografia e Estati?stica. 2012. I?ndice de Produc?a?o Pecua?ria: produc?a?o da pecua?ria municipal. Dados de 2002 a 2011. Available at: ftp://ftp.ibge.gov.br/Producao_Pecuaria/Producao_da_Pecuaria_Municipal/. Acessed 12 April 2016.

LOPES CVS, ROSA MRG, SANTOS MR, CURVELO VP, CUNHA DP, GUERRA EF, SILVA MCA, PINHEIRO DPM, CERQUEIRA RB (2011). Antibiograma como análise da sensibilidade in vitro de Corynebacterium pseudotuberculosis de caprinos. Revista acadêmica, ciências Agrárias e Ambiental; v.9 (3), 319-327.

MOTTA RC, CREMASCO, ACM, RIBEIRO, MG (2010). Infecções por Corynebacterium pseudotuberculosis em animais de produção. Veteriária e Zootecnia; v.17(2), 200-213.

MOURA-COSTA LF (2002). Corynebacteirum Pseudotuberculosis, o agente etiológico da Linfadenite Caseosa em caprinos. Revista de Ciências Médicas e Biológicas; v.1, 105- 115.

MUCKLE CA, GYLES C (1982). Characterization of strains of Corynebacterium pseudotuberculosis. Canadian Journal of Comparative Medicine; v.46, 206–208.

NASSAR AFC, DANIEL GT, RUIZ R, MIYASHIRO R, SCANNAPIECO EM, NETO JS, GREGORY L (2016). Diagnostic comparasion of Corynebacterium pseudotuberculosis through microbiological culture and in PCR sheep samples. Instituto Biológico, São Paulo, v.82, 1-6.

OSLON ME, CERI H, MORCK DW, BURET AG, READ RR (2002). Biofilm bacteria: formation and comparative susceptibility to antibiotics. Canadian Journal of Veterinary Research; v.66, 86–92.

PACHECO LGC, PENA RR, CASTRO TLP, DORELLA FA, BAHIA RC, CARMINATI R, FROTA MNL, OLIVEIA SC, MEYER R, ALVES FSF, MIYOSHI A, AZEVEDO V (2007). Multiplex PCR assay for identification of Corynebacterium pseudotuberculosis from pure cultures and for rapid detection of this pathogen in clinical samples. Journal of Medicine Microbiology;, v.56(Pt 4), 480-486.

PEEL MM, PALMER GG, STACPOOLE AM, KERR TG (1997). Human lymphadenitis due to Corynebacterium pseudotuberculosis: report of ten cases from Australia and review. Clinical Infectious Disease; v.24, 185–191.

JUNIOR JWP, OLIVEIRA AAF, ALVES FSF, SILVA LBG, RABELO SSA, MOTA RA (2006). Corynebacterium Pseudotuberculosis experimental infection of goats mamary gland. Arquivos do Instituto Biológico; v.73, p. 395-400.

RIZZO H, CARVALHO JS, FRAGA GJM, LAURIA MJS, LANGUIDEY PH (2014). Paralisia de membros pélvicos em reprodutor caprino causada por Linfadenite Caseosa no Estado de Sergipe. PUBVET, Londrina, v.8, N. 22, Ed. 271.

SANTOS AL, SANTOS DO, FREITAS CC, FERREIRA BLA, AFONSO IF, RODRIGUES CR, CASTRO HC (2007). Staphylococcus aureus: visitando uma cepa de importância hospitalar. Jornal Brasileiro de Patologia e Medicina Laboratorial; v.43(6), 413-426.

SELIM SA, GHONEIM ME, MOHAMED KHF (2010). Oedematous skin disease of buffalo in Egypt. Journal of Veterinary Medicine B Infectious Disease Vet Public Health; v.48, 241-258.

SEYFFERT N, GUIMARÃES AS, PACHECO LG, PORTELA RW, BASTOS BL, DORELLA FA, HEINEMANN MB, LAGE AP, GOUVEIA AM, MEYER R, MIYOSHI A, AZEVEDO V (2010). High seroprevalence of caseous lymphadenitis in Brazilian goat herds revealed by Corynebacterium pseudotuberculosis secreted proteins-based ELISA. Research in Veterinary Science; v.88(1), 50-55.

Published

2021-06-07

How to Cite

Alves, L. L., Almeida, D. D., Ferreira, C. de S., Brito, I., da Silva, G. A., Gaspar, L. M. do A. C., Dórea, A. C., & Padilha, F. F. (2021). Multiplex PCR for molecular characterization of isolated strains of granuloma in sheep from state of Sergipe and Antimicrobial Susceptibility Test (AST) / PCR Multiplex para caracterizaçao molecular de linhagem isolada de granuloma em ovelha no estado de Sergipe e Teste de suceptibilidade antimicrobiana. Brazilian Journal of Development, 7(5), 49131–49147. https://doi.org/10.34117/bjdv.v7i5.29869

Issue

Section

Original Papers