Aeration and Glucose Concentration on Saccharomyces boulardii Probiotic Cultivation/ Aeração e concentração de glicose no cultivo probiótico de saccharomyces boulardii

Tarcio Enrico Savoldi, Thais Lorana Savoldi, Deisy Alessandra Drunkler, Giane Andrea Linde Colauto, Nelson Barros Colauto, Mônica Lady Fiorese

Abstract


Saccharomyces boulardii viable cells have probiotic action. This study aimed to evaluate the effect of aeration on the production of S. boulardii viable cells in liquid cultivation medium with different initial glucose concentrations. Yeast has grown on yeast extract peptone dextrose medium with or without aeration (2 Lar min-1 to obtain 30 ± 1% of oxygen) and different initial glucose concentrations (20, 40 and 60 g L-1). The viable cells were determined by serial dilution method and glucose concentration was determined by 3-5 dinitrosalicylic acid. The number of viable cells changed from 7.54 ± 0.04 to 7.77 ± 0.02 log of CFU mL-1 when glucose increased from 20 to 60 g L-1, respectively. The aeration of the cultivation medium increased the number of viable cells from 7.78 ± 0.04 to 8.27 ± 0.04 log of CFU mL-1 in the cultivation medium with 40 g L-1 glucose. The yeast biomass was ~3 times greater than 60 g L-1 glucose with aeration when compared to cultivation medium with 20 g L-1 glucose without aeration. Thus, the glucose increase and the cultivation medium aeration increment S. boulardii production and viability.

As células viáveis de Saccharomyces boulardii têm ação probiótica. Este estudo teve como objetivo avaliar o efeito da aeração na produção de células viáveis de S. boulardii em meio de cultivo líquido com diferentes concentrações iniciais de glicose. A levedura cresceu em meio de peptona dextrose com extrato de levedura com ou sem aeração (2 Lar min-1 para obter 30 ± 1% de oxigênio) e diferentes concentrações iniciais de glicose (20, 40 e 60 g L-1). As células viáveis foram determinadas pelo método de diluição em série e a concentração de glicose foi determinada por 3-5 ácido dinitrosalicílico. O número de células viáveis mudou de 7,54 ± 0,04 para 7,77 ± 0,02 log de UFC UFC-1 quando a glicose aumentou de 20 para 60 g L-1, respectivamente. A aeração do meio de cultivo aumentou o número de células viáveis de 7,78 ± 0,04 para 8,27 ± 0,04 log de UFC UFC-1 no meio de cultivo com 40 g L-1 de glicose. A biomassa de levedura foi ~ 3 vezes maior que 60 g L-1 de glicose com aeração quando comparada ao meio de cultivo com 20 g L-1 de glicose sem aeração. Assim, o aumento da glicose e a aeração do meio de cultivo aumentam a produção e a viabilidade de S. boulardii.

Keywords


yeast, fungus, fermentation, cultivation, biomass, CFU

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References


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DOI: https://doi.org/10.34117/bjdv5n10-027

DOI (PDF): https://doi.org/10.34117/bjd.v5i10.3573.g3383

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