Revisão sobre a aplicação do método QuEChERS na determinação simultânea de multirresíduos (pesticidas e fármacos) presentes em alimentos de origem animal / Review on the application of the QuEChERS method in the simultaneous determination of multi-residues (pesticides and drugs) present in foods of animal origin

Cleiseano Emanuel da Silva Paniagua, Valdinei de Oliveira Santos

Abstract


Os Contaminantes de Interesse Emergente (CIEs) passaram a ser detectados e quantificados a partir da década de 1990 após o advento e o aperfeiçoamento de técnicas analíticas capazes de quantificar em escala traço (µg L-1) e ultra-traço (ng L-1) em matrizes aquosas. O crescimento desenfreado da população humana e a necessidade de aumentar a produção de alimentos, fez com que surgisse a necessidade de desenvolver métodos de extração e preparo de amostras mais versáteis, menor consumo de reagentes, maior diversidade em relação ao CIE e o tipo de amostra e que seja capaz de ser aplicado em menor tempo e em qualquer laboratório. Diante disso, em 2003 é apresentado a comunidade científica um método denominado QuEChERS (do inglês Quick, Easy, Cheap, Effective, Rugged, Safe). Tal método representou um enorme avanço em relação ao preparo e extração da amostra, permitindo analisar uma maior diversidade e quantidade de resíduos. Este trabalho tem por objetivo apresentar os fundamentos do método QuEChERS, bem como as modificações que vem sendo realizadas a fim de atingir uma maior variedade de amostras e resíduos. Além disso, apresentar e discutir inúmeros trabalhos realizados em vários países que buscam detectar e quantificar multirresíduos de pesticidas e fármacos em alimentos de origem animal e seus derivados, em especial: peixe, frangos, suínos e bovinos e os derivados ovos de frangos e leite de origem bovina.

 

 


Keywords


Contaminantes de Interesse Emergente, extração e preparo de amostras e resíduos

References


ALVES, L. T. O. et al. Síntese e caracterização de MIP com fenilalanina visando sua aplicação na técnica de SPE. Polímeros, v. 25, n. 6, p. 596-605, 2015.

AN, J. et al. Development and investigation of a QuEChERS-based method for determination of phthalate metabolites in human milk. Journal of Pharmaceutical and Biomedical Analysis,v. 181, p. 113092, 2020.

ANASTASSIADES, M. et al. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and "dispersive solid-phase extraction" for the determination of pesticide residues in produce. Journal of AOAC INTERNATIONAL, v. 86, n. 2, p. 412-431, 2003.

ARIAS, J. L. et al., Chitosan from shrimp shells: A renewable sorbent applied to the clean-up step of the QuEChERS method in order to determine multi-residues of veterinary drugs in different types of milk. Food Chemistry, v. 240, p. 1243-1253, 2018.

ARISEKAR, U. et al. Accumulation of organochlorine and pyretroid pesticide residues in fish, water, and sediments in the Thamirabarani river system of southern peninsular India. Environmental Nanotechnology, Monitoring & Management, v.11, p. 100194, 2019.

BARBIERI, M. V. et al. Analysis of 52 pesticides in fresh fish muscle by QuEChERS extraction followed by LC/MS/MS determination. Science of the Total Environmental, v. 653, p. 958-967, 2019.

BATISTA, A. P. et al. Avaliação Evaluation of the presence of ciprofloxacin, sulfamethoxazole and caffeine in sewage water from Foz do Iguaçu and Matelândia – PR. Brazilian Journal Development, v.7, n.3, p. 21374-21385, 2021.

CABRERA, L. et al. Evaluation of alternative sorbents for dispersive solid-phase extraction clean-up in the QuEChERS method for the determination of pesticides residues in rice by liquid chromatography with tandem mass spectrometry. Journal of Separation Science, v. 39, n. 10, p. 1945 – 1954, 2016.

CASTRICINI, A. et al. Determination of bifenthrin residue in bananas after harvest and in washing water. Brazilian Journal of Food Technology, v. 24, e2020118, 2021.

CASTRO, O.; POCURULL, E.; BORULL, F. Determination of organophosphate Ester flame retardants and plasticisers in fish samples by QuEChERS followed by gas chromatography-tandem mass spectrometry. Exposure and risk assessment through fish consumption. Journal of Chromatography A, v.1626, p. 461358, 2020.

CHEN, Q. et al. Quantification of 16 ?-lactams in chicken muscle by QuEChERS extraction and UPLC-Q-Orbitrap-MS with parallel reaction monitoring. Journal of Pharmaceutical and Biomedical Analysis, v. 145, p. 525-530, 2017.

CHOI, S. et al. Development and verification for analysis of pesticides in eggs and egg products using QuEChERS and LC-MS/MS. Food Chemistry, v. 173, p. 1236-1242, 2015.

CRAVEIRO, A. A.; RIBEIRO, L. M.; CRAVEIRO, A. C. Removal of pesticides in peppers and tomatoes with the use of chitosan. Brazilian Journal of Development, v. 5, n. 7, p. 10218-10227, 2019.

DASENAKI, M. E.; THOMAIDIS, N. S. Multi-residue determination of 115 veterinary drugs and pharmaceutical residues in milk powder, butter, fish tissue and eggs using liquid chromatography – tandem mass spectrometry. Analytica Chimica Acta, v. 880, p. 103-121, 2015.

DUFOUR, V. et al. Development of a simple multiresidue extraction method for the quantification of a wide polarity range list of pesticides and transformation products in eggs by liquid chromatography and tandem mass spectrometry. Journal of Chromatography A, v. 1628, p. 461447, 2020.

DYBOWSKI, M.; DAWIDOWICZ, A. Application of the QuEChERS procedure for analysis of ? 9 -tetrahydrocannabinol and its metabolites in authentic whole blood samples by GC-MS/MS. Forensic Science International, v. 36, n. 2, p. 415-423, 2018.

FEDDERN, V. et al. Ractopamine analysis in pig kidney, liver and lungs: A validation of the method scope estension using QuEChERS as a sample preparation step. Journal of Chromatography B, v. 1091, p. 79-86, 2018.

FERNANDES, V. et al. Magnetic dispersive micro solid-phase extraction and gas chromatography determination of organophosphorus pesticides in strawberries. Journal of Chromatography A, v. 1566, p. 1 – 12, 2018.

FERRONATO, G. et al. Determination of organochlorine pesticides (OCPs) in breast milk from Rio Grande do Sul, Brazil, using a modified QuEChERS method and gas chromatography-negative chemical ionization-mass spectrometry. International Journal of Environmental Analytical Chemistry, v. 98, n. 11, p. 1005 – 1016, 2018.

FENG, C. et al. Comprehensive strategy for analysis of pesticide multi-residues in food by GC-MS/MS and UPLC – Q – Orbitrap. Food Chemistry, v. 320, p. 126576, 2020.

FURLANI, R. P. Z. et al. Occurrence of macrocyclic lactones in milk and yogurt from Brazilian market. Food Control, v. 48, p. 43-47, 2015.

GABARDO, R. P. Determination of atrazine ad main metabolites in natural waters based on a simple method of QuEChERS and liquid chromatography coupled to a diode-array detector. Microchemical Journal, v. 168, p. 106392, 2021.

GUO, Q. et al. Determination of fipronil and its metabolites in chicken egg, muscle and cake by modified QuEChERS method coupled with LC-MS/MS. Food Additives & Contaminants: Part A, v. 35, n. 8, p. 1543-1552, 2018.

GUO, X. et al. Ultra-performance liquid chromatography tandem mass spectrometry for the rapid simultaneous analysis of nine organophosphate esters in milk powder. Food Chemistry, v.196, p. 673-681, 2016.

HAMED, A. et al. Evaluation of a new modified QuEChERS method for the monitoring of carbamate residues in high-fat cheeses by using UHPLC-MS/MS. Journal of Separation Science, v. 40, n.02, p. 488-496, 2016.

HAN, C. et al. Determination of Xinjunan pesticide residue in foodstuffs of plant origin by a modified QuEChERS method and ultra performance liquid chromatography-tandem mass spectrometry. LWT –Food Science and Technology, v. 151, p. 112101, 2021.

HAN, L.; SAPOZHNIKOVA, Y. Semi-automated high-throughput method for residual of 302 pesticides and environmental contaminants in catfish by fast low-pressure GC-MS/MS and UHPLC-MS/MS. Food Chemistry, v. 319, p. 126592, 2020.

HU, M. et al. Method Development and Validation of Indaziflam and its Five Metabolites in Soil, Water, and Fruits by Modified QuEChERS and UHPLC-MS/MS. Journal of Agricutural and Food Chemistry, v. 66, n. 39, p. 10300 – 10308, 2018.

KACZYNSKI, P. et al. Rapid determination of acid herbicides in soil by liquid chromatography with tandem mass spectrometric detection based on dispersive solid phase extraction. Talanta, v. 152, p. 127 – 136, 2016.

KADLIKOVA, K. et al. The investigation of honey bee pesticide poisoning incidents in Czechia. Chemosphere, v. 263, p. 128056, 2021.

LICATA, M.; et al. Hair testing in clinical setting: Simultaneous determination of 50 psychoactive drugs and metabolites in headache patients by LC tandem MS. Journal of Pharmaceutical and Biomedical Analysis, v. 15, p. 14-25, 2016.

LI, Y. N. et al. Determination of maytansinoids in Trewia nudiflora using QuEChERS. Journal of Pharmaceutical and Biomedical Analysis, v. 198, p. 113993, 2021.

LIN, Y-P. et al. Determination of multiresidue analysis of ?-agonists in muscle and víscera using liquid chromatograph/tandem mass spectrometry with Quick, Easy, Cheap, Effective, Rugged, and Safe methodologies. Journal of Food and Drug Analysis, v. 25, p. 275-284, 2017.

LIU, H. Y.; LIN, S.L.; FUH, M. R. Determination of chloramphenicol, thiamphenicol and florfenicol in milk and honey using modified QuEChERS extraction coupled with polymeric monolith-based capillary liquid chromatography tandem mass spectrometry. Talanta, v. 150, p. 233-239, 2016.

MAGALHÃES, L. S.; SOLA, M. C. Identification of aflatoxins in milk and dairy products: Literature review. Research, Society and Development, v. 10, n. 8, e50510817586, 2021.

MNYANDU, H. M.; MAHLAMBI, P. N. Optimization and application of QuEChERS and SPE methods followed by LC-PDA for the determination of triazines residues in fruits and vegetables from Pietermaritzburg local supermarkers. Food Chemistry, v. 360, p. 129818, 2021.

MONDAL, R. et al. GC-MS/MS determination and ecological risk assessment of pesticides in aquatic system: A case study in Hooghly River basin in West Bengal, India. Chemosphere, v. 206, p. 217 – 230, 2018.

MOSCHOU, I. C.; DASENAKI, M. E.; THOMAIDIS, N. S. Ionization study and simultaneous determination of avermectins and milbemcycines in fish tissue by LC-ESI-MS/MS. Journal of Chromatography B, v. 1104, p. 134-140, 2019.

MU, P. et al. Simultaneous determination of 14 antiviral drugs and relevant metabolites in chicken muscle by UPLC-MS/MS after QuEChERS preparation. Journal of Chromatography B, v. 1023-1024, p. 17-23, 2016.

PANIAGUA, C. E. S. Detecção e quantificação de resíduos de fármacos e pesticidas em águas superficiais no Brasil: Toxicologia aos organismos expostos. In: SANT’ANA, D.(org.). Água e o Ambiente Construído. Ponta Grossa: Atena Editora, v.1, n.1, p.79-89, 2021a.

PANIAGUA, C. E. S. Contribuição do método QuEChERS e das técnicas cromatográficas para consolidar os princípios da Química Verde em análises químicas. In:_____.(org.). Trabalho nas Áreas de Fronteira da Química. Ponta Grossa: Atena Editora, v. 1, n. 2, p. 140-152, 2021b.

PANIAGUA, C. E. S. Contribuição do método QuEChERS e das técnicas cromatográficas para consolidar os princípios da Química Verde em análises químicas. In:_____.(org.). Trabalho nas Áreas de Fronteira da Química. Ponta Grossa: Atena Editora, v. 1, n. 2, p. 140-152, 2021.

PANIAGUA, C. E. S.; SANTOS, V. O. Potential of using swine manure as biofertilizer, biogas and electricity: from reduced production costs to more ecologically sustainable management. Brazilian Journal of Development, v.7, n.9, p. 90227-90243, 2021.

PARK, J. A. et al. Simultaneous determination of clanobutin, dichlorvos, and naftazone in porks, beef, chicken, milk, and egg using liquid chromatography-tandem mass spectrometry. Food Chemistry, v. 252, p. 40-48, 2018.

PRESTES, O. D. et al. QuEChERS – Um método moderno de preparo de amostra para determinação multirresíduo de pesticidas em alimentos por métodos cromatográficos acoplados à espectrometria de massas. Química Nova, v. 32, n. 6, p. 1620-1634, 2009.

RUBIES, A. et al. Analysis of non-steroidal anti-inflammatory drugs in milk using QuEChERS and liquid chromatography coupled to mass spectrometry: triple quadrupole versus Q-Orbitrap mass analyzers. Analytical and Bioanalytical Chemistry, v. 408, n. 21, p. 5769 – 5778, 2016.

SAINT-HILAIRE, M. et al. Development and validation of na HPLC-MS/MS method with QuEChERS extraction using isotopic dilution to simultaneously analyze chlordecone and chlordecol in animal livers. Food Chemistry, v. 252, p. 147-153, 2018.

SAPOZHNIKOVA, Y. High-throughput analytical method for 265 pesticides and environmental contaminants in meats and poultry by fast low pressure gas chromatography tandem mass spectrometry. Journal of Chromatography A, v.1572, p. 203- 211, 2018.

SHIN, D.; KIM, J.; KANG, H. S. Simultaneous determination of multi-pesticide residues in fish and shrimp using dispersive-solid phase extraction with liquid chromatography-tandem-mass spectrometry. Food Control, v. 120, p. 107552, 2021.

SILVA, G. R. et al. Multiresidue method for identification and quantification of avermectins, benzimidazoles and nitroimidazoles residues in bovine muscle tissue by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) using a QuEChERS aprroach. Talanta, v. 171, p. 307-320, 2017.

TAN, X. T. et al. Analysis of 13 kinds of steroid hormones in raw milk using modified QuEChERS method combined with UPLC – QTOF – MS. Journal of Integrative Agriculture, v. 15, n. 9, p. 2163-2174, 2016.

TIAN, F. et al. Development of a fast multi-residue method for the determination of succinate dehydrogenase inhibitor fungicides in cereals, vegetables and fruits by modified QuEChERS and UHPLC-MS/MS. Journal of Chromatography B, v. 1152, v. 122261, 2020.

TSURUOKA, Y. et al. Siimultaneous determination of amantadine, rimantadine, and memantine in processed products, chicken tissues, and eggs by liquid chromatography with tandem mass spectrometry. Journal of Chromatography B, v. 1044-1045, p. 142-148, 2017.

UMOEKA, L. P. et al. Comparação de custo-benefício de dois métodos de extração de aflatoxinas B1, GI, B2, G2 em amostras de açúcar mascavo: Extração QuEChERS x Extração Líquido-Líquido. Revista de Iniciação Científica da ULBRA, v.1, n. 17, p. 1-12, 2019.

VARDALI, S. C.; SAMANIDOU, V. E.; KOTZAMANIS, Y. P. Development and validation of na ultra performance liquid chromatography-quadrupole time of flight-mass spectrometry (in MSE mode) method for the quantitative determination of 20 antimicrobial residues in edible muscle tissue of European sea bass. Journal of Chromatography A, v. 1575, p. 40-48, 2018.

WESTLAND, J. et al. QuEChERS extraction of benzodiazepines in biological matrices.

Journal of Pharmaceutical Analysis, v. 3, n. 6, p. 509 – 517, 2013.

XIONG, L. et al., A rapid and simple HPLC-FLD screening method with QuEChERS as the sample treatment for the simultaneous monitoring of nine bisphenols in milk. Food Chemistry, v. 244, p. 371-377, 2018.

XU, J. et al. Multiresidue analysis of 15 antibiotics in honey using modified QuEChERS and high performance liquid chromatography-tandem mass spectrometry. Journal of Food Compostion and Analysis, v. 103, p. 104120, 2021.

ZHENG, W. et al. Determination of fenobucarb residues in animal and aquatic food products using liquid chromatography-tandem mass spectrometry coupled with a QuEChERS extraction method. Journal of Chromatography B, v.1058, p. 1-7, 2017.

ZHOU, J. et al. Optimization for quick, easy, cheap, effective, rugged and safe extraction of mycotoxins and veterinary drugs by response surface methodology for application to egg and milk. Journal of Chromatography A. v. 1532, p. 20-29, 2018.

ZHOU, W. et al. Simultaneous determination of 16 macrolide antibiotics and 4 metabolites in milk by using Quick, Easy, Cheap, Effective, Rugged, and Safe extraction (QuEhERS) and high performance liquid chromatograpy tandem mass spectrometry. Journal of Chromatography B, v. 1061-1062, p. 411-420, 2017.




DOI: https://doi.org/10.34117/bjdv7n10-289

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