Evaluation of the effects of low intensity laser in proliferation of dental pulp stem cells/ Avaliação dos efeitos do laser de baixa intensidade na proliferação de células-tronco de polpa dentária

Authors

  • Patricia Yanne de Oliveira Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Leandro Marques de Resende
  • Carolina de Oliveira Lima
  • Mariane Floriano Lopes Santos Lacerda
  • Carlos Magno da Costa Maranduba
  • Antonio Marcio Resende do Carmo

DOI:

https://doi.org/10.34117/bjdv5n12-374

Keywords:

Dental pulp stem cells, low level laser, cell proliferation, laser therapy, stem cells.

Abstract

Objective: In this study, the effects of the low intensity laser were evaluated in the proliferation of human dental pulp stem cells (DPSCs). Design: These cells were irradiated every 12 hours for 72 hours or at 0 and 48 hours only, with a Red-InGaAlP laser (660nm, 30mW and 0.5 or 1J/cm2) for 16 or 33 seconds and their proliferation was assessed by the MTT assay. In addition, the Trypan Blue assay was used to analyze the viability of DPSCs in the best parameter recorded by MTT. Results: It was observed that the lowest dose of the laser (0.5J/cm2) in applications at 0 and 48 hours obtained the higher proliferation rates then all the other groups. Finally, through the Trypan Blue assay, we observed that the viability of the stem cells was not affected by the low intensity laser (0.5J/cm2). Conclusions: Our data corroborate with other data from the literature and therefore suggest that the low intensity laser can be used in order to improve cell proliferation. However, further studies should be carried out in order to evaluate if these parameters can be used in other cell lines.

References

Eduardo FP, Bueno DF, Freitas PM, Marques MM et al. Stem Cell Proliferation Under Low Intensity Laser Irradiation: A Preliminary Study. Lasers Surg Med. 2008:40:433–8.

Rodriguez-Losano FJ, Insausti CL, Iniesta F, Blanquer M et al. Mesenchymal dental stem cells in regenerative dentistry. Med Oral Patol Oral Cir Bucal. 2012:1:1062-67.

Silva FS, Almeida PN, Rettore JVP, Maranduba CP et al. Toward Personalized Cell Therapies by Using Stem Cells: Seven Relevant Topics for Safety and Success in Stem Cell Therapy. J Biomed Biotechnol. 2012:12:1-12.

Ulloa-Montoya F, Verfaillie CM, Hu WS. Culture Systems for Pluripotent Stem Cells. JBB. 2005:100:12-27.

Casagrande L, Cordeiro MM, Silva AN, Jacques EN. Dental pulp stem cells in regenerative dentistry. Society of The Nippon Dental University. 2001:99:1-7.

Demarco FF, Conde, MCM, Cavalcanti BN, Casagrande L et al. Dental Pulp Tissue Engineering. Braz Dent J. 2011:22:3-14.

Miyagi SPH, Moreira MSNA, Soares LF, Martins MM. Laserterapia e células-tronco na Odontologia. Rev Implantenews. 2012:9:53-57.

Barboza CAG, Ginani F, Soares DM, Henriques ACG et al. Laser de baixa intensidade induz à proliferação in vitro de células-tronco mesenquimais. Rev Einstein. 2014:12:75-81.

Henriques ACG, Cazal C, Castro JFL. Ação da laserterapia no processo de proliferação e diferenciação celular. Revisão da literatura. Rev. Col. Bras. Cir. 2010:37:295-302.

Ebrahimi T, Rokn AR, Heidaris M, Nokhbatolfoghahaie R et al. The Influence of Low-Intensity Laser Therapy on Bone Healing. J Dent (Tehran). 2012:9:238-48.

Emelyanov AN, Kiryanova VV. Photomodulation of Proliferation and Differentiation of Stem Cells By the Visible and Infrared Light. Photomed Laser Surg. 2015:33:164-74.

Lin F, Josephs SF, Alexandrescu D, Ramos F, Bogin V et al. Lasers, stem cells, and COPD. J Transl Med. 2010:8:1-10.

Alghandi KM, Kumar A, Moussa NM. Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci. 2012:1-13.

Azevedo LH, Eduardo FP, Moreira MS, Eduardo CP et al. Influence of different power densities of LILT on cultured human fibroblast growth - A pilot study. Lasers Med Sci. 2006: 21:86-9.

Almeida-Lopes L, Rigau J, Zangaro RA Guidugli-Neto J et al. Comparison of the low level laser therapy effects on culturehuman gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med. 2001:29:179-84.

Zaccara IM, Ginani F, Mota-Filho HG, Henriques ACG et al. Effect of low-level laser irradiation on proliferation and viability of human dental pulp stem cells. Lasers Med Sci. v. 30, n. 9, p. 2259-2264, 2015.

Pereira LO, Longo JPF, Azevedo RB. Laser irradiation did not increase the proliferation or the differentiation of stem cells from normal and inflamed dental pulp. Arch Oral Biol. 2012:57:1079-85.

Hou JF, Zhang H, Yuan X, Li J et al. In Vitro Effects of Low-Level Laser Irradiation for Bone Marrow Mesenchymal Stem Cells: Proliferation, Growth Factors Secretion and Myogenic Differentiation. Lasers Surg Med. 2008:40:726–33.

Leonida A, Paiusco A, Rossi G, Carini F et al. Effects of low-level laser irradiation on proliferation and osteoblastic differentiation of human mesenchymal stem cells seeded on a three-dimensional biomatrix: in vitro pilot study. Lasers Med Sci. 2012:1-8.

Moura-netto, C, Ferreira LS, Maranduba CM, Volpi AC et al. Low-intensity laser phototherapy enhances the proliferation of dental pulp stem cells under nutritional deficiency. Braz Oral Res. 2016:1-6.

Ginani F, Soares DM, Rocha AO e Barboza CAG. Laser de baixa intensidade promove proliferação de células-tronco derivadas de tecido adiposo criopreservadas. Rev Einstein. 2017:334-338.

Downloads

Published

2019-12-27

How to Cite

Oliveira, P. Y. de, Resende, L. M. de, Lima, C. de O., Lacerda, M. F. L. S., Maranduba, C. M. da C., & Carmo, A. M. R. do. (2019). Evaluation of the effects of low intensity laser in proliferation of dental pulp stem cells/ Avaliação dos efeitos do laser de baixa intensidade na proliferação de células-tronco de polpa dentária. Brazilian Journal of Development, 5(12), 33248–33260. https://doi.org/10.34117/bjdv5n12-374

Issue

Section

Original Papers