Chemical composition and in vitro cytotoxicity of Corynespora olivacea (V18) associated with Vochysia divergens/ Composição química e citotoxicidade in vitro de Corynespora olivacea (V18) associada a Vochysia divergens


  • Cloves Gomes de Carvalho Filho
  • Luis Claudio Kellner Filho
  • Bruno William Picão
  • Arthur Alves Ragozoni
  • Arthur Barcelos Ribeiro
  • Heloiza Diniz Nicolella
  • Iara Silva Squarisi
  • Marcos Antonio Soares
  • Denise Crispim Tavares
  • Marcio Luis Andrade e Silva
  • Wilson Roberto Cunha
  • Patricia Mendonça Pauletti
  • Ana Helena Januário



Cambará, Cerrado, Endophytic fungus, Vochysiaceae


Chemical investigation into the ethyl acetate extract of the endophytic fungus Corynespora olivacea (V18) associated with Vochysia divergens identified compounds 1 and 2. The compounds were characterized by spectroscopic methods including NMR (1D and 2D) and HRMS. The cytotoxicity of the crude extract and the isolated compounds against normal human lung fibroblast cells, lineage GM07492A, was evaluated by the XTT assay. To the best of our knowledge, this is the first chemical and biological screening of the cytotoxicity of C. olivacea derived from V. divergens.


Biz AR, Mendonça EAF, Almeida EG, Soares MA. Endophytic fungal diversity associated with the roots of cohabiting plants in the Pantanal wetland. In: Soares MA, Jardim MAG (org.) Natural resources in wetlands: from Pantanal to Amazonia, 3 rd. Museu Paraense Emílio Goeldi, Belém; 2017. p 37-70.

da Silva IP, Brissow E, Kellner Filho LC, Senabio J, de Siqueira KA, Vandresen Filho S, Damasceno JL, Mendes SA, Tavares DC, Magalhães LG, Junior PA, Januário AH, Soares MA. Bioactive compounds of Aspergillus terreus-F7, an endophytic fungus from Hyptis suaveolens (L.) Poit. World J Microbiol Biotechnol. 2017;33:62.

Dalmolin AC, Lobo FA, Vourlitis GL, Dalmagro HJ, Antunes Junior MZ, Ortiz CER. Physiological adjustments of an invasive tree species to extreme hydrological events in a tropical seasonal wetland. Trees. 2018;32:1365–1375.

de Oliveira PF, Mendes SA, Acésio NO, Filho LCK, Pimenta LP, Siqueira KA, Soares MA, Januário AH, Tavares DC. Genotoxic and Chemopreventive Effects of Vochysia divergens Leaves (Pantanal, Brazil). Evid Based Complement Alternat Med. 2018;19:6596142.

Ebrahim W, Aly AH, Mandi A, Totzke F, Kubbutat MHG, Wray V, Lin WH, Dai HF, Proksch P, Kurtan T, Debbab A. Decalactone derivatives from Corynespora cassiicola, an endophytic fungus of the mangrove plant Laguncularia racemose. Eur J Org Chem. 2012;3476-3484.

Ebrahim W, Aly AH, Wray V, Proksch P, Debbab A. Unusual octalactones from Corynespora cassiicola, an endophyte of Laguncularia racemose. Tetrahedron Lett. 2013;54:6611-6614.

Farias GC, Nunes KG, Soares MA, Siqueira KA, Lima WC, Neves ALR, Lacerda CF, Gomes Filho E. Dark septate endophytic fungi mitigate the effects of salt stress on cowpea plants. Braz J Microbiol. 2019;1:1-11.

Gonzalez MJ, Pinto MM, Kijjoa A, Kengthong S, Mondanondra IO, Silva AM, Eaton G, Herz W. 5,7-Dihydroxychromones and 8-hydroxytetrahydrochromones from Horsfieldia irya. Phytochemistry. 2002;61:995-998.

Gos F, Savi DC, Shaaban KA, Thorson JS, Aluizio R, Possiede YM, Rohr J, Glienke C. Antibacterial activity of endophytic actinomycetes isolated from the medicinal plant Vochysia divergens (Pantanal, Brazil). Front Microbiol. 2017;8:1642.

Maia VRO, Oliveira JAS, Golias HC, Pamphile JA, Polonio JC. Endophytic fungi as promoters of resistance to water and salt stress: the case of Piriformospora indica. 2020: 4:621-633.

Naik BS. Functional roles of fungal endophytes in host fitness during stress conditions. Symbiosis. 2019; 79:99–115.

Naik S., Shaanker RU, Ravikanth G, Dayanandan S. How and why do endophytes produce plant secondary metabolites? Symbiosis. 2019;78:193–201.

Noriler SA, Savi DC, Aluizio R, Palácio-Cortes AM, Possiede YM, Glienke C. Bioprospecting and structure of fungal endophyte communities found in the brazilian biomes, Pantanal, and Cerrado. Front Microbiol. 2018;24:1526.

Noriler SA, Savi DC, Ponomareva LV, Rodrigues R, Rohr J, Thorson JS, Glienke C, Shaaban KA. Vochysiamides A and B: Two new bioactive carboxamides produced by the new species Diaporthe vochysiae. Fitoterapia. 2019;138:104273.

Parpinelli BAS, Siqueira KA, Kellner LC, Pimenta LP, da Costa RM, Parreira RLT, Veneziani RCS, Silva MLAE, Cunha WR, Pauletti PM, Soares MA, Januario AH. Effect of endophytic fungal associations on the chemical profile of in vitro Vochysia divergens Seedlings. J Braz Chem Soc. 2017;28:2375-2381.

Pimenta LP, Kellner Filho LC, Rhavena GL, Soares MA, Aguiar DP, Magalhães LG, de Oliveira PF, Tavares DC, Andrade Silva Ml, Cunha WR, Pauletti PM, Januário AH. In Vitro antischistosomal activity and cytotoxicity of 5-methoxylated flavones from Vochysia divergens, a flood-adapted species from Brazilian Pantanal. Adv Pharmacoepidemiol Drug Saf. 2015;4:1-5.

Pimenta LP, Kellner Filho LC, Parpinelli BAS, Siqueira CA, Soares MA, Silva MLA, Cunha WR, Pauletti PM, Januário AH. Development and validation of an HPLC-DAD analytical method to quantify 5-methoxyflavones in methanolic extracts of Vochysia divergens pohl cultured under stress conditions. Quim Nova. 2017;40:971-975.

Savi DC, Shaaban KA, Gos FMWR, Ponomareva LV, Thorson JS, Glienke C, Rohr J. Phaeophleospora vochysiae Savi & Glienke sp. nov. Isolated from Vochysia divergens Found in the Pantanal, Brazil, Produces Bioactive Secondary Metabolites. Sci Rep. 2018;15:3122.

Savi DC, Shaaban KA, Vargas N, Ponomareva LV, Possiede YM, Thorson JS, Glienke C, Rohr J.Microbispora sp. LGMB259 endophytic actinomycete isolated from Vochysia divergens (Pantanal, Brazil) producing ?-carbolines and indoles with biological activity. Curr Microbiol. 2015;70: 345–354.

Soares MA, Li H-Y, Kowalski KP, Bergen M, Torres MS, White JF. Evaluation of the functional roles of fungal endophytes of Phragmites australis from high saline and low saline habitats. Biol Invasions. 2016;18:2689–2702.

Zhao DL, Shao CL, Gan LS, Wang M, Wang CY. Chromone derivatives from a sponge-derived strain of the fungus Corynespora cassiicola. J Nat Prod. 2015;27:286-93.





Artigos originais