Association of Ozoniotherapy to Endodontics, aiming at the tissue repair of periapical lesions - a literature review / Associação da Ozonioterapia à Endodontia, visando o reparo tecidual de lesões periapicais – uma revisão de literatura


  • Elane Lima da Silva
  • Beatriz Silveira de Morais
  • Flavia Darius Vivacqua



Ozone, dentistry, Endodontics, complementary therapy.


Introduction: The success of endodontic treatment is related to the cleaning and elimination of microorganisms and their by-products present in root canals for periapical tissue repair. Objective: To verify the efficacy of ozone (O3) as an adjunct in the therapeutic protocol of Endodontics, aiming to assist in tissue repair of periapic lesions. Methods: This is a literature review, in which scientific articles were researched, from the last 10 years, using the databases of pubmed, scielo, vhl and EBSCOhost platforms, and applying the keywords: "Ozone", "Dentistry", "Endodontics" and "Complementary therapy". Results and Discussion: The applicability of O3 is directly dependent on the performance of the well-executed endodontic treatment, to determine the best way of presenting it, increasing its effectiveness. However, during the preparation of the conduits, ozone can be used in the form of gas, being used within them, with antimicrobial purpose, highlighting the oxidative property that allows better distribution of blood flow to the tissues involved, allowing the acceleration of healing in periapic and periodontal tissues in order to stimulate tissue repair. Ozonized water can be used within the root canal, assisting in disinfection of the conduits; and ozonized oil as an intracanal dressing, both of which can be used alone or together. Conclusion: Ozone therapy is an aggregator in endodontic therapy, since it has three forms of use and several important properties to be added to the treatment, highlighting its gaseous form for assisting in the acceleration of the healing of tissue and bone repair of periapic lesions.


AGARWAL, S. et al. Comparison of antimicrobial efficacy of aqueous ozone, green tea, and normal saline as irrigants in pulpectomy procedures of primary teeth. Journal of Indian Society of Pedodontics and Preventive Dentistry, v. 8, n. 2, p. 164 - 170, 2020.

AJETI, N. N.; KRASNIQI T. P; APOSTOLSKA S. The effect of gaseous ozone in infected root canal. Open Access Macedonian Journal of Medical Sciences, v. 6, n. 2, p. 389–396, 2018.

ALMEIDA, K. O. et al. Ozonioterapia: O uso de ozônio na endodontia. Anais do Salão de Ensino e de Extensão, Universidade de Santa Cruz do Sul, p. 109, 2019.

AMIN, L. E. Biological assessment of ozone therapy on experimental oral candidiasis in immunosuppressed rats. Biochemistry and Biophysics Reports, v. 15, n. March, p. 57–60, 2018.

ANZOLIN, A. P.; BERTOL, C. D. Ozonioterapia como terapêutica integrativa no tratamento de osteoartrose: Uma revisão sistemática. Brazilian Journal of Pain, v.1, n. 2, p. 171-175, 2018.

ANZOLIN, A. P.; KAROSS S. N. L. S.; BERTOL, C. D. Ozonated oil in wound healing: What has already been proven?. Medical Gas Research, v. 10, n. 1, p. 54–59, 2020.

BARREIRA A. C. C. et al. Aspectos técnicos da ozonioterapia para odontologia: relatório técnico. Associação brasileira de ozonioterapia, 2014. Disponível em: <>.

BOCH, T. et al. Effect of gaseous ozone on Enterococcus faecalis biofilm–an in vitro study. Clinical Oral Investigations, v. 20, n. 7, p. 1733–1739, 2016.

BUKMIR, R. P. et al. A successful conservative therapy of a large periapical lesion by surgical decompression and ozone treatment: case report. Endo-Endodontic Practice Today, v. 13, n. 3, p. 265–271, 2019.

CAMPOS F. L. et al. Causas de insucessos no tratamento endodôntico - análise dos casos de retratamento atendidos no projeto de extensão da faculdade de odontologia da UFMG. Archives of Dental Science, v. 53, p.1-8, 2017.

ELVIS, A. M.; EKTA, J. S. Ozone therapy: a clinical review. Journal of Natural Science, Biology and Medicine, v. 2, n.1, p. 66-70, 2011. / 0976-9668.82319

GÓMEZ, E. M. P et al. Usos terapêuticos do ozônio em serviços de saúde. Rev. Cubana de Medicina Natural e tradicional, v. 1, n. 1, 2016.

GUPTA, S.; DEEPA, D. Applications of ozone therapy in dentistry. Journal of Oral Research and Review, n. 8, p. 86-91, 2016. / 2249-4987.192243

ISLER, S. C. et al. Effects of Laser Photobiomodulation and Ozone Therapy on Palatal Epithelial Wound Healing and Patient Morbidity. Photomedicine and Laser Surgery, v. 36, n. 11, p. 571–580, 2018.

KIST, S. et al. Comparison of ozone gas and sodium hypochlorite/chlorhexidine two-visit disinfection protocols in treating apical periodontitis: a randomized controlled clinical trial. Clinical Oral Investigations, v. 21, n. 4, p. 995–1005, 2016. / s00784-016-1849-5

LUBOJANSKI, A. et al. Application of selected nanomaterials and ozone in modern clinical dentistry. Nanomaterials, v. 11, n. 2, p. 1–30, 2021.

MAKEEVA, M. K. et al. Treatment of an endo-perio lesion with ozone gas in a patient with aggressive periodontitis: A clinical case report and literature review. Clinical, Cosmetic and Investigational Dentistry, v. 12, p. 447–464, 2020. / CCIDE.S267933

MEHTA, N. et al. Comparative evaluation of antibacterial efficacy of Allium sativum extract, aqueous ozone, diode laser, and 3% sodium hypochlorite in root canal disinfection: An in vivo study. Journal of Conservative Dentistry, v. 23, p. 577-582, 2020. 10.4103 / JCD.JCD_405_20

MELO, T. A. F. et al. LPS levels in root canals after the use of ozone gas and high frequency electrical pulses. Brazilian oral research, v. 30, p. 1–6, 2016.

MOHAMMADI Z. et al. Root canal irrigants and dentin bonding: An update. Iranian Endodontic Journal, v. 12, n. 2, p. 131–136, 2017. / iej.2017.27

MOHAMMANDI, Z.; DUMMER, P. M. H. Properties and applications of calcium hydroxide in endodontics and dental traumatology. International endodontic journal, v. 44, p. 697-730, 2011. / j.1365-2591.2011.01886.x

NAIK, S. V. et al. The Open Dentistry Journal Ozone-A Biological Therapy in Dentistry-Reality or Myth?. The Open Dentistry Journal, v. 10, p. 196–206, 2016. / 1874210601610010196

NESI, A. K. OZONIOTERAPIA: O uso do ozônio na Odontologia. Centro Universitário São Lucas, 2018.

NOGALES, C. G. et al.. Ozone therapy as an adjuvant for endondontic protocols: Microbiological – Ex vivo study and citotoxicity analyses. Journal of Applied Oral Science, v. 24, n. 6, p. 607–613, 2016. / 1678-775720160029

PAIXÃO, L. D. et al.Terapias alternativas na endodontia - ozonioterapia: revisão de literatura. Research, Society and Development, v. 10, n. 6, 2021.

PLOTINO G. et al. New Technologies to Improve Root Canal Disinfection. Brazilian Dental Journal, v. 27, n. 1, p. 3-8, 2016.

PRESTES, L. V. Aplicabilidade da ozonioterapia na odontologia: uma revisão de literatura. Arquivos de Ciências da Saúde da UNIPAR, Umuarama, v. 24, n. 3, p. 203-208, 2020.

SÁNCHEZ B. F.; SENDRA B. E. R. Aplicaciones de la ozonoterapia en la Odontología. Universidad Finis Terrae, p. 5–24, 2018. Disponível em: < 2018.pdf?sequence=1&isAllowed=y>.

SEN, S.; SEN, S. Ozone therapy a new vista in dentistry: Integrated review. Medical Gas Research, v. 10, n. 4, p. 189–192, 2020.

SILVA, E. J. N. L. et al. The effect of ozone therapy in root canal disinfection: a systematic review. International Endodontic Journal, v. 53, n. 3, p. 317–332, 2019.

SIQUEIRA, J. F. et al. Áreas de superfície do canal radicular não preparadas: causas, implicações clínicas e estratégias terapêuticas. Pesquisa Oral Brasileira n. 32, p. 65, 2018.

SUBIKSHA, P. S. Ozone and its uses in root canal therapy - A review. Journal of Pharmaceutical Sciences and Research, v. 8, n. 9, p. 1073–1076, 2016.

SUH, Y. et al. Clinical utility of ozone therapy in dental and oral medicine. Medical Gas Research, v. 9, n. 3, p. 163–167, 2019.

TIWARI, S. et al. Dental applications of ozone therapy: A review of literature. Saudi Journal for Dental Research, v. 8, n. 1–2, p. 105–111, 2017.

VASAVADA, K.; KAPOOR, S. Evaluation of ozonized calcium hydroxide as an effective intracanal medicament during root canal procedures: An in vitro observational study. Medical Gas Research, v. 10, n. 3, p. 122–124, 2020.





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