Influence of 24-epibrassinolide on the vigor of lettuce seeds / Influência do 24-epibrassinolídeo no vigor de sementes de alface

Authors

  • Júlia Gabriella da Silva Rocha Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • João Correia de Araújo Neto
  • Everton Sebastião do Nascimento
  • Mayara Rodrigues
  • Vilma Marques Ferreira
  • Clíssia Barboza da Silva

DOI:

https://doi.org/10.34117/bjdv6n7-854

Keywords:

Brassinosteroids, Lactuca sativa L, Plant Regulators, Physiological Potential.

Abstract

Seeds with low vigor often have problems to establish seedlings in the field. Studies have shown beneficial effects of 24-epibrassinolide (24-EpiBL) on seed performance, including germination speed, tolerance to heat stress, and initial development of seedlings. In this study, priming with 24-EpiBL was investigated on seed lots of lettuce with different vigor levels. Two cultivars (Florence and Betty) were tested, and each cultivar was represented by three lots. Different concentrations of 24-EpiBL were studied: 10-6, 10-8, and 10-10 M. Seeds were evaluated for germination and vigor (germination first count, germination speed index, saturated salt accelerated aging [SSAA], and seedling length).  Unprimed seeds and seeds primed with 24-EpiBL, in the best concentration identified during germination and vigor tests, were analyzed by the Seed Vigor Imaging System (SVIS®) software to generate  vigor index and growth index values. The addition of 24-EpiBL associated with priming is efficient to improve the performance of lettuce seeds. All concentrations studied were efficient, and the best was 10-6 M.

References

Ali, B., S Hayat, Q. Fariduddin, and A. Ahmad. 2008. 24-Epibrassinolide protects against the stress generated by salinity and nickel in Brassica juncea. Chemosphere, 72: 1387- 1392.

Anwar, A., L Bai, L. Miao, Y. Liu, S. Li, X. Yu and Y. Li. 2018. 24-Epibrassinolide ameliorates endogenous hormone levels to enhance low-temperature stress tolerance in cucumber seedlings. Int. J. Mol. Sci. 19: 2497.

Arora, P., R. Bhardwaj and Mk Kanwar. 2010. 24-epibrassinolide regulated diminuition of Cr metal toxicity in Brassica juncea L. plants. Braz. J. Plant Physiol, Índia, 22: 159-165.

Bajguz, A., and A. Tretyn. 2003. The chemical characteristic and distribution of brassinosteroids in plants. Phytochemistry, 62, 1027-1046.

Balbino, E., and HM Lopes. 2006. Efeitos do condicionamento fisiológico e da secagem na germinação e no vigor de sementes de cenoura. Rev. Bras. Sementes, 28: 01– 08.

Bewley JD and M Black. 1994. Seeds: physiology of development and germination. (2nd). Ed. New York: Plenum Press.

Bisognin, MB, SM Kulczynski, M. Ferrari, R. Gaviraghi, AJ Pelegrin and VQ Souza. 2016. Desempenho fisiológico de sementes olerícolas em diferentes tempos de hidrocondicionamento. Rev. Ciênc. Agrár. 39: 349-359.

Brasil, Ministério da Agricultura, Pecuária e Abastecimento – Secretaria de Defesa Agropecuária. Regras para análise de sementes. Brasília: Mapa/ACS, 2009. 399p.

Caseiro, RF, MA Bennet and J. Marcos-Filho. 2004. Comparison of three priming techniques for onion seed lots differing in initial seed quality. Seed. Sci. Technol. 32: 365-375.

Clouse, SD and JM Sasse. 1998. Brassinosteroids: essential regulators of plant growth and development. Annu. Rev. Plant. Biol. 49: 427–451.

Costa, CJ and FA Villela. 2006. Condicionamento osmótico de sementes de beterraba. Rev. Bras. Sementes, 28: 21-29.

Dalio, RJD, HP Pinheiro, L. Sodek and CRB Haddad. 2011. The effect of 24- epibrassinolide and clotrimazole on the adaptation of Cajanus cajan (L.) Millsp. to salinity. Acta Physiol. Plant,.33: 1887-1896.

Divi, UK, T. Rahman and P. Krishna. 2010. Brassinosteroid-mediated stress tolerance in Arabidopsis shows interactions with abscisic acid, ethylene and salicylic acid pathways. BMC Plant. Biol. 10: 149 -151.

Ekinci, M., E. Yildirim and A. Dursun. 2012. Mitigation of salt stress in lettuce (Lactuca sativa L. Var. Crispa) by seed and foliar 24-epibrassinolide treatments. HortScience, 47, 631-636.

El-bassiony, AM., AA Ghoname, ME El-awadi, ZF Fawzy and N. Gruda. 2012. Ameliorative effects of brassinosteroids on growth and productivity of snap beans grown under high temperature. Gesunde Pflanz. 64, 175-182.

Fessel, SA., RD Vieira, TJD Rodrigues and M. Fagioli. 2002. Germinação de sementes de alface submetidas a condicionamento osmótico durante o armazenamento. Sci. Agr. 59: 73-77.

Franzin, SM., NL Menezes, DC Garcia and CF Wrasse. 2004. Métodos para avaliação do potencial fisiológico de sementes de alface. Rev. Bras. Sementes, 26: 63-69.

Gupta, A., M. Singh, AM Jones and A. Laxmi. 2012. Hypocotyl directional growth in Arabidopsis: a complex trait. Plant physiol. 159: 1463-1476.

Hayat, S., MN Alyemeni and SA Hasan. 2012. Foliar spray of brassinosteroid enhances yield and quality of Solanum lycopersicum under cadmium stress. Saudi J. Biol. Sci. 19: 325-335.

Jianhua, Z., and MB Mcdonald. 1996. The saturated salt accelerated aging test for small-seeded crops. Seed Sci. Technol. 25: 123-131.

Kikuti, ALP and J. Marcos-filho. 2012. Testes de vigor em sementes de alface. Hortic. Bras. 30: 44-50.

Larré, CF, P. Marini, L. Moraes, L. Amarante and DM Moraes. 2014. Influência do 24-epibrassinolídeo na tolerância ao estresse salino em plântulas de arroz. Semina: Ciênc. Agrár. 35: 67-75.

Lima, LB, and J. Marcos-filho. 2010. Condicionamento fisiológico de sementes de pepino e germinação sob diferentes temperaturas. Rev. Bras. Sementes, 32: 138-147.

Maguire, JD. 1962. Speed of germination and in selection and evaluation for seedling emergence and vigor. Crop Sci. 2: 176-177.

Marcos-filho, J.2015. Fisiologia de sementes de plantas cultivadas. (2ª ed.). Londrina: Abrates.

Marcos-filho, J. and ALP Kikuti. 2008. Condicionamento fisiológico de sementes de couve-flor e desempenho das plantas em campo. Hort. Bras. 26: 165-169.

Penaloza, P., MB Mc Donald, G. Ramirez-rosales and MA Bennett. 2005. Lettuce (Lactuca sativa L.) seed quality evaluation using seed physical attributes, saturated salt accelerated aging and the seed vigour imaging system. Electron. J. Biotech. 8: 1-9

Peres, ALG, JS Soares, RG Tavares, G. Righetto, MA Zullo, NB Mandava and M. Menossi. 2019. Brassinosteroids, the sixth class of phytohormones: a molecular view from the discovery to hormonal interactions in plant development and stress adaptation. Int. J. Mol. Sci. 20: 331.

Pradhan, SK, RC Gupta and M. Kumar. 2013. Effect of 24-Epibrassinolide on lipid peroxidation and proline in three Brassica species under temperature stress. J. Stress Physiol. Biochem. 9: 376-384.

Rao, SSR, BV Vardhini, E. Sujhata and S. Anuradha. 2002. Brassinosteroids – a new class of phytohormones. Curr. Sci. 82: 1239 -1245.

Sala, FC and CP Costa. 2012. Retrospectiva e tendência da alfacicultura brasileira. Hort. Bras. 30: 187 -194.

Semida, WM and MM Rady. 2014. Pre-soaking in 24-epibrassinolide or salicylic acid improves seed germination, seedling growth, and anti-oxidant capacity in Phaseolus vulgaris L. grown under NaCl stress. J. Hortic. Sci. Biotech. 89: 338 – 344.

Serna, M., Y. Coll, PJ Zapata, MA Botella, MT Pretel and A. Amorós. 2015. A brassinosteroid analogue prevented the effect of salt stress on ethylene synthesis and polyamines in lettuce plants. Sci. Hort. 185: 105-112.

Serna, M., F. Hernández, F. Coll and A. Amorós. 2012. Brassinosteroid analogues effect on yield and quality parameters of field -grown lettuce (Lactuca sativa L.). Sci. Hort. 143: 29 -37.

Silva, CB, J. Marcos-filho, P. Jourdan and MA Bennet. 2015. Performance of Bell Pepper Seeds in Response to Drum Priming with Addition of 24-Epibrassinolide. HortScience, 50: 873 -878.

Shahid, MA, RM Balal, MA Pervez, T. Abbas, MA Aqeel, A. Riaz and NS Mattson. 2015. Exogenous 24-epibrassinolide elevates the salt tolerance potential of pea (Pisum sativum L.) by improving osmotic adjustment capacity and leaf water relations. J. Plant Nutr. 38: 1050-1072.

Shahid, MA, MA Pervez, RM Bala, NS Mattson, A. Rashid, R. Ahmad, CM Ayyub and T. Abbas. 2011. Brassinosteroid (24-epibrssinolide) enhances growth and alleviates the deleterious effects induced by salt stress in pea (Pisum sativum L.). Aust. J. Crop Sci. 5: 500-510.

Shahzad, B., M. Tanveer, Z. Che, A. Rehman, SA Cheema, A. Sharma, H. Song, S. Rehman and Zhaorong. 2018. Role of 24-epibrassinolide (EBL) in mediating heavy metal and pesticide induced oxidative stress in plants: A review. Ecotox. Environ. Safety, 147: 935-944.

Sharma, P., and R. Bhardwaj.2007. Effect of 24-epibrassinolide on seed germination, seedling growth and heavy metal uptake in Brassica Juncea L. General and Applied Plant Physiology, 33: 59-73.

Soares, TFSN, DCFDS Dias, AMS Oliveira, DM Ribeiro and LADS Dias. 2020 Brassinoesteróides exógenos aumentam a tolerância ao estresse de chumbo na germinação e crescimento de plântulas de Brassica juncea L. Ecotoxicology and Environmental Safe. 193: 110296

Vázquez, MN., YR Guerrero, LM González and WTDL Noval. 2013a. Brassinosteroids and plant responses to heavy metal stress. An overview. Open J. Met. 3: 34-41

Vázquez, MN, YR Guerrero, LR Ayan, L. Martínez, MCG Cepero and PDA Pieters.2013b. Brasinoesteroides y sus análogos estimulan el crecimiento de plántulas de dos genotipos de arroz (Oryza sativa L.) en medio salino. Cult. Trop. 34: 74-80.

Yusuf, M., Q. Fariduddin and A. Ahmad. 2012. 24-Epibrassinolide modulates growth, nodulation, antioxidant system, and osmolyte in tolerant and sensitive varieties of Vigna radiata under different levels of nickel: A shotgun approach. Plant physiol. Bioch. 57: 143-153.

Wu, X., X. Yao, J. Chen, Z. Zhu, H. Zhang and D. Zha. 2014. Brassinosteroids protect photosynthesis and antioxidant system of eggplant seedlings from high-temperature stress. Acta Physiol. Plant. 36: 251-261.

Zhang, S., J. Hu, Y. Zhang, XJ Xie and A. Knapp. 2007. Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress. Aust. J. Agri. Res. 58: 811-815.

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Published

2020-08-03

How to Cite

Rocha, J. G. da S., Neto, J. C. de A., Nascimento, E. S. do, Rodrigues, M., Ferreira, V. M., & Silva, C. B. da. (2020). Influence of 24-epibrassinolide on the vigor of lettuce seeds / Influência do 24-epibrassinolídeo no vigor de sementes de alface. Brazilian Journal of Development, 6(7), 53627–53641. https://doi.org/10.34117/bjdv6n7-854

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