Accumulation of malic acid and other industrially important organic acids by aspergillus tubingensis AN1257 / Produção biotecnológica de ácido málico por Aspergillus Tubingensis AN1257

Authors

  • Suedali Villas Bôas Coelho Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Alexandre Soares dos Santos
  • Ana Paula de Figueiredo Conte Vanzela

DOI:

https://doi.org/10.34117/bjdv6n9-519

Keywords:

Organic acids, filamentous fungi, biotechnology.

Abstract

Organic acids act as acidifiers which improve sensorial characteristics of beverages, contributing to color, aroma, and taste. Malic acid is one of the industrially important acidifiers, and is also applied in metal cleaning. There is a challenge to establish biotechnological processes for its accumulation. One approach to improve production is searching for better strains, which be adequate for bioconversion and product accumulation from cheaper substrates. The present work aimed to identify a panel of organic acids produced by Aspergillus tubingensis, which has shown high biotechnological potential due to the ability of producing a variety of enzymes and of utilizing several substrates. A. tubingensis AN1257 was compared to a standard organic acid producer, Aspergillus niger 10v10. Secretion of organic acids in dependence of the carbon source concentration, time, and pH was evaluated, and the acids produced were detected and quantified by potentiometric titration and by HPLC. Several industrially important organic acids were accumulated by this strain, including citric and malic acids, with the production being affected by culture variables. Culture pH influenced the quantity and type of acid production by strain AN1257. It was able to convert sucrose to malic acid in submerged bioprocesses, accumulating these industrially important acid in very simple media and culture conditions, posing in perspective the establishment of its biotechnological obtention.

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Published

2020-09-22

How to Cite

Coelho, S. V. B., Santos, A. S. dos, & Vanzela, A. P. de F. C. (2020). Accumulation of malic acid and other industrially important organic acids by aspergillus tubingensis AN1257 / Produção biotecnológica de ácido málico por Aspergillus Tubingensis AN1257. Brazilian Journal of Development, 6(9), 70999–71021. https://doi.org/10.34117/bjdv6n9-519

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Original Papers