Effect of nutritional factors on the Aspergillus nidulans germination program / Efeito de fatores nutricionais sobre o programa de germinação de conídios de Aspergillus nidulans
Keywords:Nutritional constituents. Filamentous fungus. Culture media. Germination of conidia.
The development of conidia germination of the filamentous fungus Aspergillus nidulans can be used to evaluate the effects of different agents. The objective of the present work was to test eight compositions of the culture medium for the germination of conidia of the bimeth strain. Dormant conidia were inoculated in minimal medium with the following compositions: TN1 (MM + glyc 1% + VIT), TN2 (MM + glyc 0.1% + VIT), TN3 (MM + glyc 1% + VIT + casein), TN4 (MM + 0.1% gli + VIT + casein), TN5 (KH2PO4 + 1% gli + VIT), TN6 (KH2PO4 + 1% gli + casein), TN7 (NaNO3 + KCl, 1% gli + VIT) and TN8 (MC). Five hundred conidia were analyzed in each observation, determining the percentage of conidia in each germination phase, for a period of up to 24 hours. After that time, the percentages of death and malformation of the conidia were determined. The results showed changes in germinative development, in the generation of dead and malformed in all TNs that lacked nutrients, when compared to MM and MC. When testing two glucose concentrations, it can be seen that glucose is not consumed during germination, although it is necessary for breaking dormancy. The absence of casein showed that the bimeth strain, which has a requirement for methionine, germinated in the absence of methionine, therefore, it was able to synthesize at least a small amount of this amino acid, although in casein media the germination was better. The absence of phosphate totally inhibited the germination of the conidia, due to the lack of raw material for DNA duplication. Some media that are poorer in nutrients than MM have shown to allow germination. The analysis of dead and malformed showed that TNs 3, 4 and 6 had a disrupting effect on polarized growth and TNs 1, 2 and 5 had a more drastic inhibitory effect, increasing cell death. The results of this work show that the conidia had changes in the development of germination in response to nutritional conditions, showing that the choice of constituents for the preparation of the medium to be used influences the responses of germination, death and malformations in conidia of A. nidulans.
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