Quercetin action on pain modulation/ Ação da quercetina sobre a modulação da dor

Authors

  • Maria Elvira Ribeiro Cordeiro Brazilian Journals Publicações de Periódicos, São José dos Pinhais, Paraná
  • Flávio Klinpovous Kerppers
  • Luiza Ferreira Cunha
  • Ketllin Bragnholo
  • Luana Rodrigues Vasconcelos
  • Andressa Panegalli Hosni
  • Ivo Ilvan Kerppers
  • Ana Carolina Dorigoni Bini
  • Felipe Figueiredo Moreira
  • Pamela Taina Licoviski
  • Patricia Pacheco Tyski Suckow
  • Angela Dubiela Julik

DOI:

https://doi.org/10.34117/bjdv7n4-697

Keywords:

quercetin, antinociception, flavonoid, pain.

Abstract

Background: Quercetin is a flavonoid widely found in plant kingdom and target of studies in pharmacological area due to its potent antinociceptive effect compared to analgesics used in conventional therapies. The aim was to evaluate its antinociceptive activity and antinociception mechanism. Methods: For this, 40 Norvegicus Wistar rats were used, divided into 4 groups: Q50 (treated with quercetin 50 mg/Kg), Q100 (treated with quercetin 100 mg/Kg), Q500 (treated with quercetin 500 mg/Kg) and Positive control (PC) without quercetin treatment), who were submitted through the pain induction methods by tail immersion and formalin in the first step to assess antinociceptive action and in the second step, tail immersion method receiving antagonists from opioid, cholinergic and nitric oxide - L-arginine to evaluate the action mechanism. Results: Quercetin antinociceptive activity was verified at the dose of 50 mg/kg and 100 mg/kg in tail immersion test after formalin injection, with better performance at the dose of 50 mg/kg. There were no statistically significant results in paw opening and capsaicin tests. Quercetin demonstrated a possible influence on opioid and cholinergic pathway, which was not observed on the nitric acid - L-arginine pathway in view of parameters tested. Conclusion: Quercetin performed the best antinociceptive activity at a dose 50 mg/kg and there was a possible influence on opioid and cholinergic pathways.

References

Resende GM, Costa ND. Produtividade e armazenamento de cebola (Allium cepa L.) submetida a doses de nitrogênio e potássio via fertirrigação em cultivo de verão. Ciênc. e Agrotec. (UFLA). 2009; 33(5), 153-163.https://doi.org/10.1590/S1413-70542009000500017.

Wu Q, Li PC, Zhang HJ, Feng CY, Li SS, Yin DD, Tian J, Xu WZ. Modelos experimentais para avaliação da atividade antinociceptiva de produtos naturais: uma revisão. Braz. J. Pharm. Sci. 2018; 94, 18-23.

Winkel-Shirley B. Flavonoid Biosynthesis: A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol. 2001; 126, 485-493.https://doi.org/10.1104/pp.126.2.485.

Willain Filho A. Potencial analgésico de flavonoides: estudo do mecanismo de ação da quercetina. Unpublished thesis, University of Vale do Itajaí. 2005.

International Association for the Study of Pain (IASP). Guia para o Tratamento da Dor em Contextos de Poucos Recursos. 2010.

Dellaroza MSG, Furuya RK, Cabrera, MAS, Matsuo T, Trelha C, Yamada KN, Pacola L. Caracterização da dor crônica e métodos analgésicos utilizados por idosos da comunidade. Rev. Ass. Med. Bras. 2008; 54(1), 36-41.

Sousa FF, Silva JA. A métrica da dor (dormetria): problemas teóricos e metodológicos. Rev. Dor. 2005; 6(1), 469-513.

Sallum, AMC, Garcia DM, Sanches M. Dor aguda e crônica: revisão narrativa da literatura. Acta Paul. de Enferm. 2012; 25(1), 150-154.https://doi.org/10.1590/S0103-21002012000800023.

Forouhar K, Pakraven DA, Khan R. Phytochemistry and pharmacological characteristics of Ruta graveolens L. 2015.

Kerppers II, Kerppers, FK, Santos KMMG, Cordeiro MER, Pereira MCS. Efeito do extrato aquoso de Cordyline Dracaenóides Kunth na cicatrização de lesões cutâneas. Medicina (Ribeirão Preto). 2019; 52(4), 267-275.https://doi.org/10.11606/issn.2176-7262.v52i4.p267-275.

Hunskaar S, Hole K. The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain. 1987; 30(1), 103-114.https://doi.org/10.1016/0304-3959(87)90088-1.

DeMedinaceli L, DeRenzo E, Wyatt RJ. Rat sciatic functional index data management system with digitized input. Comput Biomed Res. 1984; 17(2),185-192.https://doi.org/10.1016/0010-4809(84)90031-4.

Lowdon IMR, Seaber AV, Urbanlak JR. An improved method of recording rat tracks for measurement ofthe sciatic functional index of de Medinaceli. J. Neurosci. Methods. 1988; 24, 279-281. https://doi.org/10.1016/0165-0270(88)90173-2.

Kerppers II, Lima CJ, Fernandes AB, Villaverde AB. Effect of light-emitting diode (? 627 nm and 945 nm ?) treatment on first intention healing: Immunohistochemical analysis. Lasers in Med. Sci. 2015; 30, 397–401. https://doi.org/10.1007/s10103-014-1668-3.

Anjaneyulu M, Chopra K. Quercetin, a bioflavonoid, attenuates thermal hyperalgesia in a mouse model of diabetic neuropathic pain. Prog Neuropsychopharmacol Biol Psychiatry. 2003; 27, 1001-1005.https://doi.org/10.1016/S0278-5846(03)00160-X.

Martínez AL, González-Trujano ME, Aguirre-Hernández E, Moreno J, Soto-Hernández M, López-Muñoz FJ. Antinociceptive activity of Tilia americana var. mexicana inflorescences and quercetin in the formalin test and in an arthritic pain model in rats. Neuropharmacology. 2009; 56, 564–571.

Toker G, Küpeli E, Memiso?lu M, Yesilada E. Flavonoids with antinociceptive and anti-inflammatory activities from the leaves of Tilia argentea (silver linden). J. Ethnopharmacol. 2004; 95, 393–397. https://doi.org/10.1016/j.jep.2004.08.008.

Willain Filho A, Cechinel Filho V, Olinger L, Souza MM. Quercetin: Further Investigation of its Antinociceptive Properties and Mechanisms of Action. Arch Pharm Res. 2008; 31(6), 713-721.

Sousa, AM, Franco PAB, Ashmawi HA, Posso IP. Efeito analgésico local do tramadol em modelo de dor provocada por formalina em ratos. Rev. Bras. Anestesiol. 2008; 58(4), 371-379. https://doi.org/10.1590/S0034-70942008000400006.

Silva JC, Lima-Saraiva SRG, Oliveira-Júnior RG, Almeida JRGS. Modelos experimentais para avaliação da atividade antinociceptiva de produtos naturais: uma revisão. Braz. J. Pharm. Sci. 2013; 94, 18-23.

Riedel W, Neeck G. Nociception, pain, and antinociception: current concepts. Z. Rheumatol. 2001; 60, 404-415.https://doi.org/10.1007/s003930170003.

Chacur M. Efeito nociceptivo induzido por fosfolipases A2 (FLA2 variantes Lys49 e Asp49) isoladas do veneno de serpentes Bothrops asper: caracterização dos mecanismos centrais e determinantes moleculares. Unpublished thesis, University of São Paulo. 2004.

Rocha APC, Lemonica L, Barros GAM. Uso de medicações por via subaracnóidea no tratamento da dor crônica. Rev. Bras. Anestesiol. 2002; 52(5), 628–643.https://doi.org/10.1590/S0034-70942002000500014.

Golan DE, Tashjian AH, Armstrong EJ. Princípios de Farmacologia - a Base Fisiopatológica da Farmacoterapia. 2009. 2nd ed. Guanabara Koogan.

Bassanezi BSB, Oliveira Filho AG de. Analgesia pós-operatória. Rev Col Bras Cir. 2006; 33(2), 116-122. http://dx.doi.org/10.1590/S0100-69912006000200012.

Ribeiro PG. Avaliação do efeito antinociceptivo em diferentes vias de administração e da interação farmacológica com receptores opioides e canabinoides da protonectina-F. Unpublished thesis, University of Brasília. 2019.

Martins RT, Almeida DB, Monteiro FMR, Kowacs PA, Ramina R. Receptores opioides até o contexto atual. Rev. Dor. 2012; 13(1), 75-79. http://dx.doi.org/10.1590/S1806-00132012000100014.

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Published

2021-04-30

How to Cite

Cordeiro, M. E. R., Kerppers, F. K., Cunha, L. F., Bragnholo, K., Vasconcelos, L. R., Hosni, A. P., Kerppers, I. I., Bini, A. C. D., Moreira, F. F., Licoviski, P. T., Suckow, P. P. T., & Julik, A. D. (2021). Quercetin action on pain modulation/ Ação da quercetina sobre a modulação da dor. Brazilian Journal of Development, 7(4), 43616–43634. https://doi.org/10.34117/bjdv7n4-697

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Original Papers